Phospho-MSP/MST1 (Thr353) Antibody - #AF3688

Figure S2. Expanded rat skin was divided into well-regenerated and poorly regenerated groups, similar to regenerated human skin, and Fps were found to be widely distributed in both the upper papillary dermis and the lower reticular dermis. (a) Representative photographs of well-regenerated and poorly regenerated expanded rat skin. (b‒e) Histochemical assessments of (b, d) PCNA+ cells and (c, e) YAP+ cells in the rat dermis from the indicated groups and their quantification. n = 8. (f, g) Representative images showing DLK1 and LRIG1 costaining in (f) the papillary dermis and (g) the reticular dermis in each group. (h) LRIG1+ cells were widely distributed in the area surrounding the perifollicular dermis. The white dashed line shows hair follicles. (i) Representative images of TGFβ1 staining in the papillary dermis and the reticular dermis in each group. (j) Representative costained images of TGFβ1 and LRIG1 staining in well-regenerated rat skin. (k) Representative αSMA-stained images from each group. Bar = 100 μm. Data are presented as the mean ± SEM. One-way ANOVA followed by Tukey’s multiple comparison test was used. ∗P < 0.05, ∗∗P < 0.01. The results are representative of at least three independent experiments. CON denotes normal skin (control group), WELL denotes well-regenerated skin, POOR denotes poorly regenerated skin, and OLD denotes aged skin. αSMA, α-smooth muscle actin; Fp, papillary fibroblast; n.s., nonsignificant; PCNA, proliferating cell nuclear antigen.

Fig. 3. Effect of ICA on Hippo signaling pathway in BMSCs (A- B) Relative mRNA expression levels of MST1 and TAZ (n = 3); (C–G) Relative protein expression levels of MST1, p-MST1, TAZ and p-TAZ (n = 3).