Phospho-MST1 (Thr353) Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse heart, using Phospho-MST1 (Thr353) Antibody.

Product:	Phospho-MST1 (Thr353) Antibody
Catalog:	AF3688
Description:	Rabbit polyclonal antibody to Phospho-MST1 (Thr353)
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	80kD(Calculated).
Uniprot:	P26927
RRID:	AB_2847002

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-MST1 (Thr353) Antibody detects endogenous levels of MST1 only when phosphorylated at Thr353.

RRID:

AB_2847002
Cite Format: Affinity Biosciences Cat# AF3688, RRID:AB_2847002.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

D3F15S2; DNF15S2; Hepatocyte growth factor like protein alpha chain; Hepatocyte growth factor like protein; Hepatocyte growth factor like protein beta chain; Hepatocyte growth factor like protein homolog; Hepatocyte growth factor-like protein beta chain; HGFL; HGFL_HUMAN; Macrophage stimulating 1 (hepatocyte growth factor like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MST1; NF15S2; OTTHUMP00000208927;

Immunogens

Immunogen:

A synthesized peptide derived from human MST1 around the phosphorylation site of Thr353.

Uniprot:

P26927(HGFL_HUMAN) >>Visit HPA database.

Gene(ID):

MST1(4485)

Sequence:

P26927 HGFL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGWLPLLLLLTQCLGVPGQRSPLNDFQVLRGTELQHLLHAVVPGPWQEDVADAEECAGRCGPLMDCRAFHYNVSSHGCQLLPWTQHSPHTRLRRSGRCDLFQKKDYVRTCIMNNGVGYRGTMATTVGGLPCQAWSHKFPNDHKYTPTLRNGLEENFCRNPDGDPGGPWCYTTDPAVRFQSCGIKSCREAACVWCNGEEYRGAVDRTESGRECQRWDLQHPHQHPFEPGKFLDQGLDDNYCRNPDGSERPWCYTTDPQIEREFCDLPRCGSEAQPRQEATTVSCFRGKGEGYRGTANTTTAGVPCQRWDAQIPHQHRFTPEKYACKDLRENFCRNPDGSEAPWCFTLRPGMRAAFCYQIRRCTDDVRPQDCYHGAGEQYRGTVSKTRKGVQCQRWSAETPHKPQFTFTSEPHAQLEENFCRNPDGDSHGPWCYTMDPRTPFDYCALRRCADDQPPSILDPPDQVQFEKCGKRVDRLDQRRSKLRVVGGHPGNSPWTVSLRNRQGQHFCGGSLVKEQWILTARQCFSSCHMPLTGYEVWLGTLFQNPQHGEPSLQRVPVAKMVCGPSGSQLVLLKLERSVTLNQRVALICLPPEWYVVPPGTKCEIAGWGETKGTGNDTVLNVALLNVISNQECNIKHRGRVRESEMCTEGLLAPVGACEGDYGGPLACFTHNCWVLEGIIIPNRVCARSRWPAVFTRVSVFVDWIHKVMRLG

PTMs - P26927 As Substrate

P26927

Site	PTM Type	Source
S185	Phosphorylation	Uniprot
Y199	Phosphorylation	Uniprot
N296	N-Glycosylation	Uniprot
S480	Phosphorylation	Uniprot
K573	Ubiquitination	Uniprot
N615	N-Glycosylation	Uniprot

PTMs - P26927 As Enzyme

P26927

Substrate	Site	Source
P33778 (HIST1H2BB)	S15	Uniprot
Q13043 (STK4)	T183	Uniprot
Q15208 (STK38)	T444	Uniprot

Research Backgrounds

P26927_HGFL_HUMAN

PTMs:

Cleaved after Arg-483, probably by HPN/Hepsin, to yield the active form consisting of two disulfide-linked chains.

Subcellular Location:

Secreted.

Subunit Structure:

Dimer of an alpha chain and a beta chain linked by a disulfide bond. Interacts (via beta chain) with MST1R (via SEMA domain).

Family&Domains:

Belongs to the peptidase S1 family. Plasminogen subfamily.

References

1). Mechanical stretching can modify the papillary dermis pattern and papillary fibroblast characteristics during skin regeneration. Journal of Investigative Dermatology (PubMed: 35181299) [IF=6.5]

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Phospho-MST1 (Thr353) Antibody - #AF3688