Phospho-XIRP1 (Ser295) Antibody - #AF3762

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Product: Phospho-XIRP1 (Ser295) Antibody
Catalog: AF3762
Description: Rabbit polyclonal antibody to Phospho-XIRP1 (Ser295)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 110kDa; 123kD(Calculated).
Uniprot: O70373
RRID: AB_2847076

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-XIRP1 (Ser295) Antibody detects endogenous levels of XIRP1 only when phosphorylated at Ser295.
RRID:
AB_2847076
Cite Format: Affinity Biosciences Cat# AF3762, RRID:AB_2847076.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

A synthesized peptide derived from mouse XIRP1 around the phosphorylation site of Ser295.

Uniprot:

O70373(XIRP1_MOUSE) >>Visit HPA database.

Expression:
O70373 XIRP1_MOUSE:

Expressed in heart and skeletal muscle. In heart, present in intercalated disks (at protein level).

Sequence:
MADAQMQVAPTPTIQMRTEEDLSLPHPSAPEGLPPPPPKETFSKFQQQRQASELRRLYKHIHPELRKNLEEAVAEDLAEVLGSEEPTEGDVQCMRWIFENWRLDAIGDHERPAAREPVSGGNVQATSRKFEEGSFTNSSDQEPEGLRPSGGDVQAARQMFETKPLDALRGQEEATQTTMREPAATGDVQGTRKLFETRPLDRLGSRPSIQEQSPLELRSEIQELKGDVKKTVKLFQTEPLCAIQDAEGTIHEVKAACREEIQSNAVRSARWLFETRPLDAFNQDPSQVRVIRGISLEEGALPDVSATRWIFETQPLDAIREIEVDEKDFQPSPDLIPPGPDVQHQRHLFETCSLDTLKGERETEAEVPPKEEVIPGDVRSTLWLFETKPLDAFRDQVQVGHLQRVGHQEGEGLVTECLPSNGTSVLPLSQGVPQNDGLKGDVKTFKNLFETLPLDSIGQGEPSAYGNINRGQNTDSAEQSQGSDAPVYAMQDSRGQLHALTSVSREQVVGGDVQGYKWMFETQPLDTLGRSPSTIDVVRGITRQEVVAGDVGTTRWLFETQPLEMIHQQEQQKPEEEEGKGPGGPPPELPKKGDVQTIRWLFETYPMSELAEKRESEVTDPVSKAETQSCTWMFGPQSLNPAEGSGEQHLQTSQVPAGDRQTDRHVFETESLPASNQSSGRKPVRYCSRVEIPSGQVSRQKEVFQALEAGKKEVPETTINLGSIPTGSVHKFTWLFENCPMGSLAAESIRGDNLQEEQPKGSAGHGTPERQETAAERTLRTLHATPGILHHGGILMEARGPGELCLAKYVLPSPGQGRPYIRKEELVCGELPRIVRQVLRRTDVDQQGLLVQEDTAGQLQLHPLMLPGPGDPGNIEDMDPELQQLLACGLGVSVSKTGLVMQETGQGLVALTAYSLQPQLTSRAPERSSVQLLASCIDKGDLHSLHSLRWEPPTDPSSGPATEESQRVPPTESIIHVTPLDSTMEMGQLRISGSTPCPPPSRAAGKVVLPNGKPVAQAPLQEARKKRDISHAGQKGKAASGRPEASPLGSGAPDLQEAMQNLRLATAEAQSLHQQVLSRHPQGSDPVATSMPVQDVLQASTPATGVTQGSIRPVAGSEARIPAVPRKLL

Research Backgrounds

Function:

Protects actin filaments from depolymerization.

Subcellular Location:

Cell junction.
Note: Colocalizes with actin stress fibers.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart and skeletal muscle. In heart, present in intercalated disks (at protein level).

Subunit Structure:

Interacts with FLNC and VASP (By similarity). Interacts with F-actin and CTNNB1.

Family&Domains:

Xin repeats bind F-actin.

Belongs to the Xin family.

Restrictive clause

 

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