Phospho-eEF2K (Ser491/Ser492) Antibody

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Phospho-eEF2K (Ser491/Ser492) Antibody - Western blot analysis of extracts from 3T3-L1 cells(heat-shock treatment), using Phospho-eEF2K (Ser491/Ser492) Antibody.

Phospho-eEF2K (Ser491/Ser492) Antibody - AF3785 at 1/100 staining Rat liver tissue by IHC-P.

Phospho-eEF2K (Ser491/Ser492) Antibody - AF3785 at 1/100 staining Rat brain tissue by IHC-P.

Product:	Phospho-eEF2K (Ser491/Ser492) Antibody
Catalog:	AF3785
Description:	Rabbit polyclonal antibody to Phospho-eEF2K (Ser491/Ser492)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	82kD(Calculated).
Uniprot:	O00418
RRID:	AB_2847099

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-eEF2K (Ser491/Ser492) Antibody detects endogenous levels of eEF2K only when phosphorylated at Ser491/Ser492.

RRID:

AB_2847099
Cite Format: Affinity Biosciences Cat# AF3785, RRID:AB_2847099.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C86191; Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent protein kinase III; cb365; EC 2.7.11.20; eEF 2 kinase; eEF 2K; eEF-2 kinase; eEF-2K; Eef2k; EEF2K protein; EF2K_HUMAN; Elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; fa04b08; HSU93850; kinase eEF2K; MGC45041; SMEF2K; wu:fa04b08; zgc:100973;

Immunogens

Immunogen:

A synthesized peptide derived from human eEF2K around the phosphorylation site of Ser491/Ser492.

Uniprot:

O00418(EF2K_HUMAN) >>Visit HPA database.

Gene(ID):

EEF2K(29904)

Sequence:

O00418 EF2K_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Research Backgrounds

O00418_EF2K_HUMAN

Function:

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

PTMs:

Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.

Family&Domains:

Belongs to the protein kinase superfamily. Alpha-type protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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Phospho-eEF2K (Ser491/Ser492) Antibody - #AF3785