Phospho-n-Myc (Ser261/Ser263) Antibody - #AF3881

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Product: Phospho-n-Myc (Ser261/Ser263) Antibody
Catalog: AF3881
Description: Rabbit polyclonal antibody to Phospho-n-Myc (Ser261/Ser263)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 50kD(Calculated).
Uniprot: P04198
RRID: AB_2847195

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-n-Myc (Ser261/Ser263) Antibody detects endogenous levels of n-Myc only when phosphorylated at Ser261/263.
RRID:
AB_2847195
Cite Format: Affinity Biosciences Cat# AF3881, RRID:AB_2847195.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHe37; Class E basic helix-loop-helix protein 37; MODED; mycn; MYCN_HUMAN; N myc; N myc proto oncogene protein; N-myc proto-oncogene protein; Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene; Neuroblastoma MYC oncogene; NMYC; NMYC proto oncogene protein; ODED; Oncogene NMYC; pp65/67; V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog; v myc avian myelocytomatosis viral related oncogene neuroblastoma derived; v myc myelocytomatosis viral related oncogene neuroblastoma derived;

Immunogens

Immunogen:

A synthesized peptide derived from human n-Myc around the phosphorylation site of Ser261/263.

Uniprot:

P04198(MYCN_HUMAN) >>Visit HPA database.

Gene(ID):
MYCN(4613)
Expression:
P04198 MYCN_HUMAN:

Expressed in the neuronal cells of the cerebrum, neuroblastomas and thyroid tumors (at protein level).

Sequence:
MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC

PTMs - P04198 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
S5 Phosphorylation
T6 Phosphorylation
S7 Phosphorylation
T8 Phosphorylation
K52 Ubiquitination
T58 Phosphorylation
S62 Phosphorylation P42345 (MTOR)
S64 Phosphorylation
R65 Methylation
K133 Acetylation
S156 Phosphorylation
R238 Methylation
R242 Methylation
S261 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S263 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
K321 Ubiquitination
S355 Phosphorylation

Research Backgrounds

Function:

Positively regulates the transcription of MYCNOS in neuroblastoma cells.

PTMs:

Phosphorylated by GSK3-beta which may promote its degradation. Phosphorylated by AURKA.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the neuronal cells of the cerebrum, neuroblastomas and thyroid tumors (at protein level).

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with KDM5A, KDM5B and HUWE1. Interacts with MYCNOS. Interacts with AURKA; interaction is phospho-independent and triggers AURKA activation; AURKA competes with FBXW7 for binding to unphosphorylated MYCN but not for binding to unphosphorylated MYCN. Interacts with FBXW7; FBXW7 competes with AURKA for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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