Product: Thrombin Receptor Antibody
Catalog: AF0263
Description: Rabbit polyclonal antibody to Thrombin Receptor
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 48kDa; 47kD(Calculated).
Uniprot: P25116
RRID: AB_2833437

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Thrombin Receptor Antibody detects endogenous levels of total Thrombin Receptor.
RRID:
AB_2833437
Cite Format: Affinity Biosciences Cat# AF0263, RRID:AB_2833437.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CF2R; Coagulation factor II (thrombin) receptor; Coagulation factor II receptor; F2R; HTR; PAR 1; PAR-1; PAR1; PAR1_HUMAN; Protease activated receptor 1; Proteinase activated receptor 1; Proteinase-activated receptor 1; Thrombin receptor; TR;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P25116 PAR1_HUMAN:

Platelets and vascular endothelial cells.

Description:
PAR1 a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.
Sequence:
MGPRRLLLVAACFSLCGPLLSARTRARRPESKATNATLDPRSFLLRNPNDKYEPFWEDEEKNESGLTEYRLVSINKSSPLQKQLPAFISEDASGYLTSSWLTLFVPSVYTGVFVVSLPLNIMAIVVFILKMKVKKPAVVYMLHLATADVLFVSVLPFKISYYFSGSDWQFGSELCRFVTAAFYCNMYASILLMTVISIDRFLAVVYPMQSLSWRTLGRASFTCLAIWALAIAGVVPLLLKEQTIQVPGLNITTCHDVLNETLLEGYYAYYFSAFSAVFFFVPLIISTVCYVSIIRCLSSSAVANRSKKSRALFLSAAVFCIFIICFGPTNVLLIAHYSFLSHTSTTEAAYFAYLLCVCVSSISCCIDPLIYYYASSECQRYVYSILCCKESSDPSSYNSSGQLMASKMDTCSSNLNNSIYKKLLT

PTMs - P25116 As Substrate

Site PTM Type Enzyme
K51 Ubiquitination
Y52 Phosphorylation
Y69 Phosphorylation
S306 Phosphorylation
S375 Phosphorylation
S376 Phosphorylation
Y383 Phosphorylation
S384 Phosphorylation
S391 Phosphorylation
S392 Phosphorylation
S395 Phosphorylation
S396 Phosphorylation
Y397 Phosphorylation
S399 Phosphorylation
S400 Phosphorylation
S406 Phosphorylation
K407 Ubiquitination
T410 Phosphorylation
S412 Phosphorylation
S413 Phosphorylation
S418 Phosphorylation
Y420 Phosphorylation
K421 Ubiquitination
K422 Ubiquitination
T425 Phosphorylation

Research Backgrounds

Function:

High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

PTMs:

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Phosphorylated in the C-terminal tail; probably mediating desensitization prior to the uncoupling and internalization of the receptor.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Platelets and vascular endothelial cells.

Family&Domains:

The cleaved signal peptide may not be degraded and may function as an intracellular angiogenesis inhibitor peptide known as parstatin.

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

References

1). Zhao Y et al. Inhibiting trophoblast PAR-1 overexpression suppresses sFlt-1-induced anti-angiogenesis and abnormal vascular remodeling: a possible therapeutic approach for preeclampsia. MOLECULAR HUMAN REPRODUCTION 2018 Mar 1;24(3):158-169 (PubMed: 29325127) [IF=4.0]

2). Yu M et al. pHLIP(Var7)-P1AP suppresses tumor cell proliferation in MDA-MB-231 triple-negative breast cancer by targeting protease activated receptor 1. BREAST CANCER RESEARCH AND TREATMENT 2020 Feb 7 (PubMed: 32034579) [IF=3.8]

Application: IF/ICC    Species: Human    Sample: MDA-MB-231 and MCF-10A cells

Fig. 1 PAR1 expression was positive in MDA-MB-231 cells, and PAR1 expression was negative in MCF-10A cells (400 ×)

3). Zhong W et al. Doxycycline directly targets PAR1 to suppress tumor progression. Oncotarget 2017 Mar 7;8(10):16829-16842 (PubMed: 28187433)

Application: IF/ICC    Species: human    Sample:

(G) Confocal immunofluorescence imaging was performed to visualize PAR1 and doxycycline. PAR1 and doxycycline co-localize, with a Pearson’s correlation coeffcient of 0.944 and a Mander’s overlap of 0.964.

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