Product: TIF1 alpha Antibody
Catalog: AF6774
Description: Rabbit polyclonal antibody to TIF1 alpha
Application: WB
Reactivity: Human
Mol.Wt.: 80,100,120kD; 74kD(Calculated).
Uniprot: Q9NYV6
RRID: AB_2847497

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
TIF1 alpha Antibody detects endogenous levels of total TIF1 alpha.
RRID:
AB_2847497
Cite Format: Affinity Biosciences Cat# AF6774, RRID:AB_2847497.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AL023001; DKFZp566E104; E130302O19Rik; MGC104238; R75565; RGD1305001; RNA polymerase I-specific transcription initiation factor RRN3; Rrn3; RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae); RRN3_HUMAN; TIF-IA; TIF1A; TIFIA; Transcription Initiation Factor 1A; Transcription initiation factor IA; Transcription initiation factor TIF IA;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAAPLLHTRLPGDAAASSSAVKKLGASRTGISNMRALENDFFNSPPRKTVRFGGTVTEVLLKYKKGETNDFELLKNQLLDPDIKDDQIINWLLEFRSSIMYLTKDFEQLISIILRLPWLNRSQTVVEEYLAFLGNLVSAQTVFLRPCLSMIASHFVPPRVIIKEGDVDVSDSDDEDDNLPANFDTCHRALQIIARYVPSTPWFLMPILVEKFPFVRKSERTLECYVHNLLRISVYFPTLRHEILELIIEKLLKLDVNASRQGIEDAEETATQTCGGTDSTEGLFNMDEDEETEHETKAGPERLDQMVHPVAERLDILMSLVLSYMKDVCYVDGKVDNGKTKDLYRDLINIFDKLLLPTHASCHVQFFMFYLCSFKLGFAEAFLEHLWKKLQDPSNPAIIRQAAGNYIGSFLARAKFIPLITVKSCLDLLVNWLHIYLNNQDSGTKAFCDVALHGPFYSACQAVFYTFVFRHKQLLSGNLKEGLQYLQSLNFERIVMSQLNPLKICLPSVVNFFAAITNKYQLVFCYTIIERNNRQMLPVIRSTAGGDSVQICTNPLDTFFPFDPCVLKRSKKFIDPIYQVWEDMSAEELQEFKKPMKKDIVEDEDDDFLKGEVPQNDTVIGITPSSFDTHFRSPSSSVGSPPVLYMQPSPL

PTMs - Q9NYV6 As Substrate

Site PTM Type Enzyme
R9 Methylation
S19 Phosphorylation
K22 Ubiquitination
S44 Phosphorylation P24941 (CDK2)
K62 Ubiquitination
K65 Ubiquitination
K75 Ubiquitination
S170 Phosphorylation P68400 (CSNK2A1)
S172 Phosphorylation P68400 (CSNK2A1)
S199 Phosphorylation
T200 Phosphorylation P45984 (MAPK9)
S233 Phosphorylation
K253 Ubiquitination
T271 Phosphorylation
S319 Phosphorylation
K334 Ubiquitination
K415 Ubiquitination
K480 Ubiquitination
K568 Ubiquitination
K593 Ubiquitination
K598 Ubiquitination
K610 Ubiquitination
T623 Phosphorylation
S633 Phosphorylation P27361 (MAPK3)
S635 Phosphorylation Q13131 (PRKAA1)
S636 Phosphorylation
S640 Phosphorylation
S649 Phosphorylation P51812 (RPS6KA3) , P27361 (MAPK3)

Research Backgrounds

Function:

Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction (By similarity).

PTMs:

Phosphorylation is required for participation in rDNA transcription (By similarity). Phosphorylated at Thr-200 by MAPK9/JNK2, which abrogates initiation complex formation.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with TWISTNB, EIF3L, TAF1B and TAF1C.

Family&Domains:

Belongs to the RRN3 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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