HBP1 Antibody | Affinity Biosciences

WB
IF/ICC

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HBP1 Antibody - Western blot analysis of extracts from A549 cells(H2O2 treatment), using HBP1 Antibody.

HBP1 Antibody - AF6844 staining A549 cells by IF/ICC.

Product:	HBP1 Antibody
Catalog:	AF6844
Description:	Rabbit polyclonal antibody to HBP1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	58kD(Calculated).
Uniprot:	O60381
RRID:	AB_2847567

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

HBP1 Antibody detects endogenous levels of total HBP1.

RRID:

AB_2847567
Cite Format: Affinity Biosciences Cat# AF6844, RRID:AB_2847567.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ16340; HBP 1; HBP1; HBP1_HUMAN; High mobility group box transcription factor 1; HMG box containing protein 1; HMG box transcription factor 1; HMG box-containing protein 1;

Immunogens

Immunogen:

A synthesized peptide derived from human HBP1.

Uniprot:

O60381(HBP1_HUMAN) >>Visit HPA database.

Gene(ID):

HBP1(26959)

Sequence:

O60381 HBP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVWEVKTNQMPNAVQKLLLVMDKRASGMNDSLELLQCNENLPSSPGYNSCDEHMELDDLPELQAVQSDPTQSGMYQLSSDVSHQEYPRSSWNQNTSDIPETTYRENEVDWLTELANIATSPQSPLMQCSFYNRSSPVHIIATSKSLHSYARPPPVSSSSKSEPAFPHHHWKEETPVRHERANSESESGIFCMSSLSDDDDLGWCNSWPSTVWHCFLKGTRLCFHKGSNKEWQDVEDFARAEGCDNEEDLQMGIHKGYGSDGLKLLSHEESVSFGESVLKLTFDPGTVEDGLLTVECKLDHPFYVKNKGWSSFYPSLTVVQHGIPCCEVHIGDVCLPPGHPDAINFDDSGVFDTFKSYDFTPMDSSAVYVLSSMARQRRASLSCGGPGGQDFARSGFSKNCGSPGSSQLSSNSLYAKAVKNHSSGTVSATSPNKCKRPMNAFMLFAKKYRVEYTQMYPGKDNRAISVILGDRWKKMKNEERRMYTLEAKALAEEQKRLNPDCWKRKRTNSGSQQH

PTMs - O60381 As Substrate

O60381

Site	PTM Type	Enzyme	Source
K16	Ubiquitination		Uniprot
K23	Ubiquitination		Uniprot
S135	Phosphorylation		Uniprot
S156	Phosphorylation		Uniprot
S157	Phosphorylation		Uniprot
S158	Phosphorylation		Uniprot
S159	Phosphorylation		Uniprot
K171	Acetylation		Uniprot
K225	Ubiquitination		Uniprot
K229	Ubiquitination		Uniprot
K255	Ubiquitination		Uniprot
K263	Ubiquitination		Uniprot
K297	Acetylation		Uniprot
K297	Ubiquitination		Uniprot
K305	Acetylation		Uniprot
K305	Ubiquitination		Uniprot
K307	Acetylation		Uniprot
S372	Phosphorylation		Uniprot
S380	Phosphorylation		Uniprot
S382	Phosphorylation		Uniprot
K398	Ubiquitination		Uniprot
S402	Phosphorylation	Q15759 (MAPK11) , Q16539 (MAPK14)	Uniprot
K416	Ubiquitination		Uniprot
K419	Acetylation		Uniprot
K419	Ubiquitination		Uniprot
S430	Phosphorylation		Uniprot
K433	Ubiquitination		Uniprot
T453	Phosphorylation		Uniprot
T484	Phosphorylation		Uniprot
K488	Ubiquitination		Uniprot
K495	Ubiquitination		Uniprot
K503	Ubiquitination		Uniprot
S509	Phosphorylation		Uniprot

Research Backgrounds

O60381_HBP1_HUMAN

Function:

Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the histone H1.0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.

PTMs:

Ubiquitinated by the CTLH E3 ubiquitin-protein ligase complex, leading to subsequent proteasomal degradation.

Subcellular Location:

Nucleus.

Subunit Structure:

Binds the second PAH repeat of SIN3A (Probable). Binds TCF4. Binds RB1.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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HBP1 Antibody - #AF6844