Product: LRRK2 Antibody
Catalog: AF6887
Description: Rabbit polyclonal antibody to LRRK2
Application: WB
Reactivity: Human
Mol.Wt.: 286kD(Calculated).
Uniprot: Q5S007
RRID: AB_2847610

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
LRRK2 Antibody detects endogenous levels of total LRRK2.
RRID:
AB_2847610
Cite Format: Affinity Biosciences Cat# AF6887, RRID:AB_2847610.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

augmented in rheumatoid arthritis 17; AURA17; Dardarin; Leucine rich repeat kinase 2; leucine rich repeat serine threonine protein kinase 2; Leucine-rich repeat serine/threonine-protein kinase 2; LRRK 2; LRRK2; LRRK2_HUMAN; PARK 8; PARK8; RIPK7; ROCO 2; ROCO2;

Immunogens

Immunogen:

A synthesized peptide derived from human LRRK2.

Uniprot:
Gene(ID):
Expression:
Q5S007 LRRK2_HUMAN:

Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed in neutrophils (at protein level) (PubMed:29127255). Expressed in the brain. Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.

Sequence:
MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE

PTMs - Q5S007 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation Q5S007 (LRRK2)
S5 Phosphorylation Q5S007 (LRRK2)
T358 Phosphorylation
T424 Phosphorylation Q5S007 (LRRK2)
T489 Phosphorylation
T496 Phosphorylation
T524 Phosphorylation Q5S007 (LRRK2)
S633 Phosphorylation
S634 Phosphorylation
Y636 Phosphorylation
Y707 Phosphorylation
T776 Phosphorylation Q5S007 (LRRK2)
S784 Phosphorylation
S788 Phosphorylation
T826 Phosphorylation Q5S007 (LRRK2)
T833 Phosphorylation Q5S007 (LRRK2)
S837 Phosphorylation
T838 Phosphorylation Q5S007 (LRRK2)
S850 Phosphorylation
S858 Phosphorylation
S860 Phosphorylation
S865 Phosphorylation
S895 Phosphorylation
S898 Phosphorylation
S908 Phosphorylation
S910 Phosphorylation O14920 (IKBKB) , Q9UHD2 (TBK1) , O15111 (CHUK) , Q14164 (IKBKE) , P17612 (PRKACA)
S912 Phosphorylation
S926 Phosphorylation
S933 Phosphorylation
S935 Phosphorylation O15111 (CHUK) , Q14164 (IKBKE) , Q9UHD2 (TBK1) , P17612 (PRKACA) , O14920 (IKBKB)
S954 Phosphorylation
S955 Phosphorylation
S958 Phosphorylation
S962 Phosphorylation
S971 Phosphorylation
S973 Phosphorylation
S975 Phosphorylation
S976 Phosphorylation
S979 Phosphorylation
T1024 Phosphorylation
S1025 Phosphorylation
S1058 Phosphorylation
K1118 Ubiquitination
S1124 Phosphorylation Q5S007 (LRRK2)
K1129 Ubiquitination
S1157 Phosphorylation
S1159 Phosphorylation
T1176 Phosphorylation
S1219 Phosphorylation
S1228 Phosphorylation
S1253 Phosphorylation Q5S007 (LRRK2)
S1283 Phosphorylation Q5S007 (LRRK2)
S1292 Phosphorylation Q5S007 (LRRK2)
Y1332 Phosphorylation Q5S007 (LRRK2)
T1343 Phosphorylation Q5S007 (LRRK2)
S1345 Phosphorylation Q5S007 (LRRK2)
T1348 Phosphorylation Q5S007 (LRRK2)
T1349 Phosphorylation Q5S007 (LRRK2)
T1357 Phosphorylation Q5S007 (LRRK2)
T1368 Phosphorylation Q5S007 (LRRK2)
Y1402 Phosphorylation Q5S007 (LRRK2)
S1403 Phosphorylation Q5S007 (LRRK2)
T1404 Phosphorylation Q5S007 (LRRK2)
T1410 Phosphorylation Q5S007 (LRRK2)
S1443 Phosphorylation Q5S007 (LRRK2)
S1444 Phosphorylation Q5S007 (LRRK2) , P17612 (PRKACA)
S1445 Phosphorylation Q5S007 (LRRK2)
T1452 Phosphorylation Q5S007 (LRRK2)
S1457 Phosphorylation Q5S007 (LRRK2)
S1467 Phosphorylation Q5S007 (LRRK2)
T1470 Phosphorylation Q5S007 (LRRK2)
Y1485 Phosphorylation Q5S007 (LRRK2)
T1491 Phosphorylation Q5S007 (LRRK2)
T1503 Phosphorylation Q5S007 (LRRK2)
S1508 Phosphorylation Q5S007 (LRRK2)
S1536 Phosphorylation Q5S007 (LRRK2)
T1612 Phosphorylation Q5S007 (LRRK2)
S1627 Phosphorylation Q00535 (CDK5)
S1647 Phosphorylation Q5S007 (LRRK2)
Y1718 Phosphorylation
S1721 Phosphorylation
K1833 Ubiquitination
T1849 Phosphorylation Q5S007 (LRRK2)
S1853 Phosphorylation Q5S007 (LRRK2)
T1912 Phosphorylation Q5S007 (LRRK2)
S1913 Phosphorylation Q5S007 (LRRK2)
T1967 Phosphorylation Q5S007 (LRRK2)
T1969 Phosphorylation Q5S007 (LRRK2)
Y2023 Phosphorylation
T2031 Phosphorylation Q5S007 (LRRK2)
S2032 Phosphorylation Q5S007 (LRRK2)
T2035 Phosphorylation Q5S007 (LRRK2)
S2166 Phosphorylation
T2237 Phosphorylation
S2257 Phosphorylation Q5S007 (LRRK2)
Y2449 Phosphorylation
T2460 Phosphorylation
T2483 Phosphorylation Q5S007 (LRRK2)
T2524 Phosphorylation Q5S007 (LRRK2)

PTMs - Q5S007 As Enzyme

Substrate Site Source
O15379 (HDAC3) S424 Uniprot
O95295 (SNAPIN) T117 Uniprot
P04637 (TP53) T304 Uniprot
P04637 (TP53) T377 Uniprot
P10636-8 (MAPT) T149 Uniprot
P10636-8 (MAPT) T153 Uniprot
P10636-8 (MAPT) T205 Uniprot
P15311 (EZR) T567 Uniprot
P26038 (MSN) T558 Uniprot
P30048 (PRDX3) T146 Uniprot
P31749 (AKT1) S473 Uniprot
P35241 (RDX) T564 Uniprot
P37840 (SNCA) S129 Uniprot
P46459 (NSF) T645 Uniprot
P61020 (RAB5B) T6 Uniprot
P62280 (RPS11) T28 Uniprot
P62280 (RPS11) T46 Uniprot
P62280 (RPS11) T54 Uniprot
P62841 (RPS15) T136 Uniprot
Q13541 (EIF4EBP1) T37 Uniprot
Q13541 (EIF4EBP1) T46 Uniprot
Q14155 (ARHGEF7) T127 Uniprot
Q14155 (ARHGEF7) T164 Uniprot
Q16665 (HIF1A) S797 Uniprot
Q5S007 (LRRK2) S3 Uniprot
Q5S007 (LRRK2) S5 Uniprot
Q5S007 (LRRK2) T424 Uniprot
Q5S007 (LRRK2) T524 Uniprot
Q5S007 (LRRK2) T776 Uniprot
Q5S007 (LRRK2) T826 Uniprot
Q5S007 (LRRK2) T833 Uniprot
Q5S007 (LRRK2) T838 Uniprot
Q5S007 (LRRK2) S1124 Uniprot
Q5S007 (LRRK2) S1253 Uniprot
Q5S007 (LRRK2) S1283 Uniprot
Q5S007 (LRRK2) S1292 Uniprot
Q5S007 (LRRK2) Y1332 Uniprot
Q5S007 (LRRK2) T1343 Uniprot
Q5S007 (LRRK2) S1345 Uniprot
Q5S007 (LRRK2) T1348 Uniprot
Q5S007 (LRRK2) T1349 Uniprot
Q5S007 (LRRK2) T1357 Uniprot
Q5S007 (LRRK2) T1368 Uniprot
Q5S007 (LRRK2) Y1402 Uniprot
Q5S007 (LRRK2) S1403 Uniprot
Q5S007 (LRRK2) T1404 Uniprot
Q5S007 (LRRK2) T1410 Uniprot
Q5S007 (LRRK2) S1443 Uniprot
Q5S007 (LRRK2) S1444 Uniprot
Q5S007 (LRRK2) S1445 Uniprot
Q5S007 (LRRK2) T1452 Uniprot
Q5S007 (LRRK2) S1457 Uniprot
Q5S007 (LRRK2) S1467 Uniprot
Q5S007 (LRRK2) T1470 Uniprot
Q5S007 (LRRK2) Y1485 Uniprot
Q5S007 (LRRK2) T1491 Uniprot
Q5S007 (LRRK2) T1503 Uniprot
Q5S007 (LRRK2) S1508 Uniprot
Q5S007 (LRRK2) S1536 Uniprot
Q5S007 (LRRK2) T1612 Uniprot
Q5S007 (LRRK2) S1647 Uniprot
Q5S007 (LRRK2) T1849 Uniprot
Q5S007 (LRRK2) S1853 Uniprot
Q5S007 (LRRK2) T1912 Uniprot
Q5S007 (LRRK2) S1913 Uniprot
Q5S007 (LRRK2) T1967 Uniprot
Q5S007 (LRRK2) T1969 Uniprot
Q5S007 (LRRK2) T2031 Uniprot
Q5S007 (LRRK2) S2032 Uniprot
Q5S007 (LRRK2) T2035 Uniprot
Q5S007 (LRRK2) S2257 Uniprot
Q5S007 (LRRK2) T2483 Uniprot
Q5S007 (LRRK2) T2524 Uniprot
Q99962 (SH3GL2) S75 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, autophagy, and vesicle trafficking. Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation. Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43. Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A. Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A. Regulates primary ciliogenesis through phosphorylation of RAB8A and RAB10, which promotes SHH signaling in the brain. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose-6-phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Plays an important role in recruiting SEC16A to endoplasmic reticulum exit sites (ERES) and in regulating ER to Golgi vesicle-mediated transport and ERES organization. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Phosphorylates PRDX3. By phosphorylating APP on 'Thr-743', which promotes the production and the nuclear translocation of the APP intracellular domain (AICD), regulates dopaminergic neuron apoptosis. Independent of its kinase activity, inhibits the proteosomal degradation of MAPT, thus promoting MAPT oligomerization and secretion. In addition, has GTPase activity via its Roc domain which regulates LRRK2 kinase activity.

PTMs:

Autophosphorylated. Phosphorylation of Ser-910 and either Ser-935 or Ser-1444 facilitates interaction with YWHAG. Phosphorylation of Ser-910 and/or Ser-935 facilitates interaction with SFN.

Subcellular Location:

Cytoplasmic vesicle. Perikaryon. Golgi apparatus membrane>Peripheral membrane protein. Cell projection>Axon. Cell projection>Dendrite. Endoplasmic reticulum membrane>Peripheral membrane protein. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane>Peripheral membrane protein.
Note: Colocalized with RAB29 along tubular structures emerging from Golgi apparatus (PubMed:23395371). Localizes to endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER) (PubMed:25201882).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed in neutrophils (at protein level). Expressed in the brain. Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.

Subunit Structure:

Homodimer. Interacts with PRKN, PRDX3, and TPCN2. Interacts with VPS35 and RAB29. Interacts (via ROC domain) with SEC16A. Interacts with APP; interaction promotes phosphorylation of 'Thr-743' of APP. Interacts with MAPT. Interacts with RAB8A, RAB10, and RAB12. Interacts with YWHAG; this interaction is dependent on phosphorylation of Ser-910 and either Ser-935 or Ser-1444. Interacts with SFN; this interaction is dependent on phosphorylation of Ser-910 and/or Ser-935.

Family&Domains:

The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins (PubMed:24687852). It also mediates homodimerization and regulates kinase activity (PubMed:30635421).

The Roc domain mediates homodimerization and regulates kinase activity.

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

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