Product: AKAP8 Antibody
Catalog: DF3025
Description: Rabbit polyclonal antibody to AKAP8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 76 KD; 76kD(Calculated).
Uniprot: O43823
RRID: AB_2835056

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 100ul $280 In stock
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Product Info

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
AKAP8 Antibody detects endogenous levels of total AKAP8.
Cite Format: Affinity Biosciences Cat# DF3025, RRID:AB_2835056.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


A kinase (PRKA) anchor protein 8; A kinase anchor protein 8; A kinase anchor protein 95; A kinase anchor protein 95 kDa; A kinase anchor protein 95kDa; A-kinase anchor protein 8; A-kinase anchor protein 95 kDa; AKAP 8; AKAP 95; AKAP-8; AKAP-95; Akap8; AKAP8_HUMAN; AKAP95; DKFZp586B1222;



Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells.




Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43823 As Substrate

Site PTM Type Enzyme
Y51 Phosphorylation P12931 (SRC) , P07948 (LYN)
Y53 Phosphorylation P07948 (LYN) , P12931 (SRC)
Y80 Phosphorylation P07948 (LYN) , P12931 (SRC)
K95 Ubiquitination
R109 Methylation
S112 Phosphorylation
S136 Phosphorylation
Y146 Phosphorylation P07948 (LYN) , P12931 (SRC)
S149 Phosphorylation
Y150 Phosphorylation P07948 (LYN) , P12931 (SRC)
Y152 Phosphorylation P12931 (SRC) , P07948 (LYN)
Y154 Phosphorylation P07948 (LYN) , P12931 (SRC)
S165 Phosphorylation
Y170 Phosphorylation P12931 (SRC) , P07948 (LYN)
S182 Phosphorylation
R188 Methylation
R190 Methylation
R194 Methylation
R198 Methylation
S199 Phosphorylation
Y229 Phosphorylation
R233 Methylation
R242 Methylation
R277 Methylation
R281 Methylation
T301 Phosphorylation
K306 Ubiquitination
Y311 Phosphorylation P07948 (LYN) , P12931 (SRC)
K317 Ubiquitination
S323 Phosphorylation
S328 Phosphorylation
S339 Phosphorylation
C351 S-Nitrosylation
S353 Phosphorylation
S399 Phosphorylation
K407 Ubiquitination
T423 Phosphorylation
K424 Ubiquitination
Y436 Phosphorylation P07948 (LYN) , P12931 (SRC)
K465 Ubiquitination
K517 Ubiquitination
K524 Ubiquitination
K534 Acetylation
K538 Acetylation
Y539 Phosphorylation P12931 (SRC) , P07948 (LYN)
S644 Phosphorylation
T681 Phosphorylation
S685 Phosphorylation

Research Backgrounds


Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L. Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation. May be involved in regulation of DNA replication by acting as scaffold for MCM2. Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation. May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells. May act as a carrier protein of GJA1 for its transport to the nucleus. Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters. Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity).


Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes.

Subcellular Location:

Nucleus. Nucleus matrix. Nucleus>Nucleolus. Cytoplasm.
Note: Associated with the nuclear matrix in interphase and redistributes mostly to chromatin at mitosis. However, mitotic chromatin localization has been questioned. Upon nuclear reassembly at the end of mitosis, is sequestered into the daughter nuclei where it re-acquires an interphase distribution. Localized to the nucleolus in interphase. Colocalizes with GJA1 at the nuclear membrane specifically during cell cycle G1/S phase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells.

Subunit Structure:

Binds to the PKA RII-alpha regulatory subunit PRKAR2A (phosphorylated at 'Thr-54') during mitosis. Interacts (via C-terminus) with FIGN (By similarity). Interacts with NCAPD2, CCND1, MCM2, RPS6KA1, PDE4A. Interacts with CCND3, CCNE1, DDX5, CASP3. Interacts with NFKB1; detetcted in the cytoplasm. Interacts with MYCBP; MYCBP is translocated to the nucleus and the interaction prevents the association of the PKA catalytic subunit leading to suppression of PKA activity (By similarity). Interacts with DPY30; mediating AKAP8 association with at least the MLL4/WBP7 HMT complex. Interacts with HDAC3; increased during mitosis. Interacts with GJA1; in the nucleus and in the nuclear membrane; the nuclear association increases with progress of cell cycle G1, S and G2 phase and decreases in M phase.


Belongs to the AKAP95 family.

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