Lamin B1 Mouse Monoclonal Antibody | Affinity Biosciences

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Lamin B1 Mouse Monoclonal Antibody - Western blot analysis of extracts from various samples, using Lamin B1 Mouse Monoclonal Antibody.

Lamin B1 Mouse Monoclonal Antibody - .

Lamin B1 Mouse Monoclonal Antibody - Figure 6.

Lamin B1 Mouse Monoclonal Antibody - Fig.

Lamin B1 Mouse Monoclonal Antibody - Figure 3.

Lamin B1 Mouse Monoclonal Antibody - Fig.

Product:	Lamin B1 Mouse Monoclonal Antibody
Catalog:	BF8009
Description:	Mouse monoclonal antibody to Lamin B1
Application:	WB
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	66kD; 66kD(Calculated).
Uniprot:	P20700

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$150	In stock
100ul	$250	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8009]

Specificity:

Lamin B1 Mouse Monoclonal Antibody detects endogenous levels of total Lamin B1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1_HUMAN; MGC111419; OTTHUMP00000159218;

Immunogens

Immunogen:

A synthesized peptide derived from human Lamin B1.

Uniprot:

P20700(LMNB1_HUMAN) >>Visit HPA database.

Gene(ID):

LMNB1(4001)

Sequence:

P20700 LMNB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATATPVPPRMGSRAGGPTTPLSPTRLSRLQEKEELRELNDRLAVYIDKVRSLETENSALQLQVTEREEVRGRELTGLKALYETELADARRALDDTARERAKLQIELGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLADETLLKVDLENRCQSLTEDLEFRKSMYEEEINETRRKHETRLVEVDSGRQIEYEYKLAQALHEMREQHDAQVRLYKEELEQTYHAKLENARLSSEMNTSTVNSAREELMESRMRIESLSSQLSNLQKESRACLERIQELEDLLAKEKDNSRRMLTDKEREMAEIRDQMQQQLNDYEQLLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRVDVEESEASSSVSISHSASATGNVCIEEIDVDGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYVLKAGQTVTIWAANAGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTIPEEEEEEEEAAGVVVEEELFHQQGTPRASNRSCAIM

Research Backgrounds

P20700_LMNB1_HUMAN

Function:

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

PTMs:

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

Subcellular Location:

Nucleus inner membrane>Lipid-anchor>Nucleoplasmic side.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

References

1). A tumor-derived mitochondrial tsRNA drives vascular invasion of lung adenocarcinoma by promoting ribosomal assembly in endothelial cells. Cancer letters, 2026 (PubMed: 41707977) [IF=9.1]

2). ANXA1 promotes tumor immune evasion by binding PARP1 and upregulating Stat3-induced expression of PD-L1 in multiple cancers. Cancer Immunology Research, 2023 (PubMed: 37566399) [IF=8.1]

3). Honokiol suppresses the aberrant interactions between renal resident macrophages and tubular epithelial cells in lupus nephritis through the NLRP3/IL-33/ST2 axis. Cell Death & Disease, 2023 (PubMed: 36859530) [IF=8.1]

Application: WB Species: Mouse Sample:

Fig. 5: Effects of Honokiol (HNK) on the renal levels of the NLRP3 inflammasome and the ST2 and NF-κB proteins in MpJ/lpr mice.

4). WLJP-025p, a homogeneous Lonicera japonica polysaccharide, attenuates atopic dermatitis by regulating the MAPK/NFκB/AP-1 axis via Act1. International journal of biological macromolecules, 2024 (PubMed: 38016605) [IF=7.7]

5). Ginsenoside Rg2 ameliorates metabolic dysfunction-related steatohepatitis via the Nrf2 pathway by suppressing inflammation, apoptosis, oxidative stress and fibrosis. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2025 (PubMed: 41308396) [IF=6.7]

6). Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis. Journal of orthopaedic translation, 2024 (PubMed: 39007035) [IF=5.9]

7). Improving hyperglycemic effect of FGF-21 is associated with alleviating inflammatory state in diabetes. International Immunopharmacology, 2018 (PubMed: 29414665) [IF=4.8]

8). Panax notoginseng Saponins Activate Nuclear Factor Erythroid 2-Related Factor 2 to Inhibit Ferroptosis and Attenuate Inflammatory Injury in Cerebral Ischemia-Reperfusion. The American journal of Chinese medicine, 2024 (PubMed: 38699996) [IF=4.8]

9). FGF-21 Elevated IL-10 Production to Correct LPS-Induced Inflammation. INFLAMMATION, 2018 (PubMed: 29427162) [IF=4.5]

10). Astragaloside IV mitigates cerebral ischaemia-reperfusion injury via inhibition of P62/Keap1/Nrf2 pathway-mediated ferroptosis. European Journal of Pharmacology, 2023 (PubMed: 36758783) [IF=4.2]

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Lamin B1 Mouse Monoclonal Antibody - #BF8009