Lamin B1 Mouse Monoclonal Antibody - #BF8009

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Product: Lamin B1 Mouse Monoclonal Antibody
Catalog: BF8009
Description: Mouse monoclonal antibody to Lamin B1
Application: WB
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 66kD; 66kD(Calculated).
Uniprot: P20700

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Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8009]
Specificity:
Lamin B1 Mouse Monoclonal Antibody detects endogenous levels of total Lamin B1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1_HUMAN; MGC111419; OTTHUMP00000159218;

Immunogens

Immunogen:

A synthesized peptide derived from human Lamin B1.

Uniprot:

P20700(LMNB1_HUMAN) >>Visit HPA database.

Gene(ID):
LMNB1(4001)
Sequence:
MATATPVPPRMGSRAGGPTTPLSPTRLSRLQEKEELRELNDRLAVYIDKVRSLETENSALQLQVTEREEVRGRELTGLKALYETELADARRALDDTARERAKLQIELGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLADETLLKVDLENRCQSLTEDLEFRKSMYEEEINETRRKHETRLVEVDSGRQIEYEYKLAQALHEMREQHDAQVRLYKEELEQTYHAKLENARLSSEMNTSTVNSAREELMESRMRIESLSSQLSNLQKESRACLERIQELEDLLAKEKDNSRRMLTDKEREMAEIRDQMQQQLNDYEQLLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRVDVEESEASSSVSISHSASATGNVCIEEIDVDGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYVLKAGQTVTIWAANAGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTIPEEEEEEEEAAGVVVEEELFHQQGTPRASNRSCAIM

Research Backgrounds

Function:

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

PTMs:

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

Subcellular Location:

Nucleus inner membrane>Lipid-anchor>Nucleoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

References

1). Honokiol suppresses the aberrant interactions between renal resident macrophages and tubular epithelial cells in lupus nephritis through the NLRP3/IL-33/ST2 axis. Cell Death & Disease, 2023 (PubMed: 36859530) [IF=8.1]

Application: WB    Species: Mouse    Sample:

Fig. 5: Effects of Honokiol (HNK) on the renal levels of the NLRP3 inflammasome and the ST2 and NF-κB proteins in MpJ/lpr mice.
2). ANXA1 promotes tumor immune evasion by binding PARP1 and upregulating Stat3-induced expression of PD-L1 in multiple cancers. Cancer Immunology Research, 2023 (PubMed: 37566399) [IF=8.1]
3). WLJP-025p, a homogeneous Lonicera japonica polysaccharide, attenuates atopic dermatitis by regulating the MAPK/NFκB/AP-1 axis via Act1. International journal of biological macromolecules, 2024 (PubMed: 38016605) [IF=7.7]
4). Improving hyperglycemic effect of FGF-21 is associated with alleviating inflammatory state in diabetes. International Immunopharmacology, 2018 (PubMed: 29414665) [IF=4.8]
5). Panax notoginseng Saponins Activate Nuclear Factor Erythroid 2-Related Factor 2 to Inhibit Ferroptosis and Attenuate Inflammatory Injury in Cerebral Ischemia-Reperfusion. The American journal of Chinese medicine, 2024 (PubMed: 38699996) [IF=4.8]
6). FGF-21 Elevated IL-10 Production to Correct LPS-Induced Inflammation. INFLAMMATION, 2018 (PubMed: 29427162) [IF=4.5]
7). Astragaloside IV mitigates cerebral ischaemia-reperfusion injury via inhibition of P62/Keap1/Nrf2 pathway-mediated ferroptosis. European Journal of Pharmacology, 2023 (PubMed: 36758783) [IF=4.2]
8). Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. FOOD AND CHEMICAL TOXICOLOGY, 2017 (PubMed: 28137608) [IF=3.9]

Application: WB    Species: rat    Sample: PC12 cells

9). Echinatin mitigates sevoflurane-induced hippocampal neurotoxicity and cognitive deficits through mitigation of iron overload and oxidative stress. PHARMACEUTICAL BIOLOGY, 2022 (PubMed: 36205592) [IF=3.9]

Application: WB    Species: Rat    Sample:

Figure 3. Ech activates Nrf2 in hippocampal neurons and hippocampus of aged rats. (A and B) The mRNA and protein levels of Nrf2 in vitro were detected by qRT-PCR and western blot. (C and D) The mRNA and protein levels of Nrf2 in vivo were detected by qRT-PCR and western blot. Compared with the Sev group
10). Interaction with tumor‑associated macrophages promotes PRL‑3‑induced invasion of colorectal cancer cells via MAPK pathway‑induced EMT and NF‑κB signaling‑induced angiogenesis. ONCOLOGY REPORTS, 2019 (PubMed: 30864736) [IF=3.8]

Application: WB    Species: mouse    Sample: LoVo‑P and HT29‑NC cells

Figure 6.| PRL‑3 promotes angiogenesis by activating the NF‑κB pathway.(D) NF‑κB pathway activation was examined by western blot analysis. *P<0.05. PRL‑3, phosphatase of regenerating liver‑3; VEGF, vascular endothelial growth factor; TAM, tumor‑associated macrophage; NC, negative control; LoVo‑P, LoVo PRL‑3 overexpression; p, phosphor; IKKα, nuclear factor κ‑B kinase subunit α; AREG, amphiregulin; b‑NGF, β nerve growth factor; EGF, epidermal growth factor; EGFR, epidermal growth factor receptor; GM‑CSF, granulocyte‑macrophage colony‑stimulating factor; IGFBP2, insulin‑like growth factor‑binding protein 2; NT‑4, neurotrophin‑4; VEGFR2, vascular endothelial growth factor receptor 2; IκBα, NF‑κB inhibitor α.
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