MYOCD Antibody | Affinity Biosciences

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MYOCD Antibody - FIGURE 3 MBNL1 increases Myocardin expression by binding to UGCU at the 3'-UTR of Myocardin mRNA.

MYOCD Antibody - Fig.

Product:	MYOCD Antibody
Catalog:	AF0023
Description:	Rabbit polyclonal antibody to MYOCD
Application:	WB IHC IF/ICC
Cited expt.:	WB, IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	100 kDa; 102kD(Calculated).
Uniprot:	Q8IZQ8

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

MYOCD Antibody detects endogenous levels of total MYOCD.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MYCD; MYCD_HUMAN; Myocardin; Myocd;

Immunogens

Immunogen:

A synthesized peptide derived from human MYOCD, corresponding to a region within the internal amino acids.

Uniprot:

Q8IZQ8(MYCD_HUMAN) >>Visit HPA database.

Gene(ID):

MYOCD(93649)

Expression:

Q8IZQ8 MYCD_HUMAN:

Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.

Description:

Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.

Sequence:

Q8IZQ8 MYCD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW

Research Backgrounds

Q8IZQ8_MYCD_HUMAN

Function:

Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).

PTMs:

Phosphorylation regulates negatively the intrinsic myocardin transcriptional activity.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.

Family&Domains:

The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation (By similarity).

References

1). MBNL1 regulates isoproterenol‐induced myocardial remodelling in vitro and in vivo. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33295096) [IF=5.3]

Application: WB Species: mice Sample: cardiomyocytes

FIGURE 3 MBNL1 increases Myocardin expression by binding to UGCU at the 3'-UTR of Myocardin mRNA. A, The 3'-UTR region of Myocardin contains the binding site of MBNL1. B, The binding of MBNL1 to Myocardin mRNA was validated using RIP. (n = 3, *, P < .05). C-F, The changes in Myocardin and MBNL1 in cardiomyocytes with overexpressed or silenced MBNL1 were measured using realtime PCR and Western blotting. (n = 3, *, P < .05, **, P < .01). G and H, Myocardin mRNA residues after 0, 20, 40 and 60 min in cardiomyocytes treated with Actinomycin D (ACD, 5 μg/mL) and with overexpressed or silenced MBNL1were measured using realtime PCR. I, The 3'-UTR region of Myocardin mRNA was transcribed in vitro and labelled with biotin. The combination of MBNL1 protein and 3'-UTR of Myocardin was detected using RNA pulldown assay. J, The RNA probes corresponding to nine prediction sites (probe # 10-18) and their corresponding antisense probes (probe # 1-9) were synthesized and biotin-labelled. The combination site of MBNL1 protein in the 3'-UTR of Myocardin was confirmed using RNA pull-down assay

2). LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2020 (PubMed: 31982428) [IF=4.9]

Application: WB Species: mouse Sample: primary cardiomyocytes

Fig. 2. |Mhrt regulates myocardin expression through KLF4 in primary cardiomyocytes.(D, E and F) Following knock down of endogenous KLF4 in primary cardiomyocytes, the effect of Mhrt on myocardin mRNA and protein levels was examined (n = 3, *,P < .05, **, P < .01, #, P > .05).

3). Myocardin Reverses Hypoxia-Inducible Factor-1α Mediated Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells in Hypoxia Induced by. World Journal of Mens Health, 2023 (PubMed: 35274501) [IF=4.0]

4). Myocardin Reverses Hypoxia-Inducible Factor-1α Mediated Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells in Hypoxia Induced by Cobalt Chloride. The world journal of men's health, 2023 (PubMed: 35274501) [IF=4.0]

Application: IF/ICC Species: human Sample: CCSMCs

Fig. 3. Phenotypic modulation in CCSMCs under hypoxia. (A) Representative images of immunofluorescence detecting HIF-1α, Myocd and α-SMA. Scale bar=100 µm. (B) Quantitative results of the statistical analysis. (C) Representative western blotting results of HIF-1α, Myocd, α-SMA, calponin, and OPN. (D) Quantitative results of the statistical analysis. n=3 for each group. CCSMCs: corporal cavernosum smooth muscle cells, HIF-1α: hypoxia-inducible factor-1α, α-SMA: alpha-smooth muscle actin, Myocd: myocardin, OPN: osteopontin. *p

Application: WB Species: human Sample: CCSMCs

5). KLHL38 facilitates STS‐induced apoptosis in HL‐1 cells via myocardin degradation. IUBMB LIFE, 2022 (PubMed: 35112472) [IF=3.7]

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MYOCD Antibody - #AF0023