Product: CDK7 Antibody
Catalog: AF0267
Description: Rabbit polyclonal antibody to CDK7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 39kDa; 39kD(Calculated).
Uniprot: P50613
RRID: AB_2833441

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
CDK7 Antibody detects endogenous levels of total CDK7.
Cite Format: Affinity Biosciences Cat# AF0267, RRID:AB_2833441.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


39 kDa protein kinase; CAK; CAK1; Cdk activating kinase; CDK-activating kinase 1; CDK-activating kinase; cdk7; CDK7_HUMAN; CDKN7; Cell division protein kinase 7; Cyclin dependent kinase 7; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); Cyclin-dependent kinase 7; HCAK; Homolog of Xenopus MO15 Cdk activating kinase; Kinase subunit of CAK; MO15; MO15, Xenopus, homolog of; P39 Mo15; p39MO15; Serine threonine kinase Stk1; Serine/threonine protein kinase 1; Serine/threonine protein kinase MO15; STK1; TFIIH basal transcription factor complex kinase subunit;


P50613 CDK7_HUMAN:


CDK7 a protein kinase of the CDK family. Forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). Activates the cyclin-associated kinases CDK1, -2, -4 and -6. An essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. Serves as a direct link between the regulation of transcription and the cell cycle



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50613 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K6 Ubiquitination
S7 Phosphorylation
T25 Phosphorylation
K32 Ubiquitination
K41 Ubiquitination
S106 Phosphorylation
T110 Phosphorylation
K139 Ubiquitination
K160 Ubiquitination
S161 Phosphorylation Q9HC98 (NEK6)
S164 Phosphorylation P50613 (CDK7) , P24941 (CDK2) , P06493 (CDK1)
Y169 Phosphorylation
T170 Phosphorylation P50613 (CDK7) , P41743 (PRKCI) , P06493 (CDK1) , P24941 (CDK2)
T175 Phosphorylation
S217 Phosphorylation
T287 Phosphorylation
K291 Ubiquitination
T316 Phosphorylation
K318 Ubiquitination
S321 Phosphorylation
K328 Ubiquitination
K330 Ubiquitination
T332 Phosphorylation

PTMs - P50613 As Enzyme

Substrate Site Source
P03372 (ESR1) S118 Uniprot
P04637 (TP53) S33 Uniprot
P04637 (TP53) S371 Uniprot
P04637 (TP53) S376 Uniprot
P04637 (TP53) S378 Uniprot
P04637 (TP53) S392 Uniprot
P06493 (CDK1) T161 Uniprot
P10275 (AR) S516 Uniprot
P10276 (RARA) S77 Uniprot
P11802 (CDK4) T172 Uniprot
P13631 (RARG) S77 Uniprot
P13631 (RARG) S79 Uniprot
P14859 (POU2F1) S385 Uniprot
P21127 (CDK11B) T595 Uniprot
P24928 (POLR2A) S1616 Uniprot
P24928 (POLR2A) S1619 Uniprot
P24928 (POLR2A) S1621 Uniprot
P24928 (POLR2A) S1626 Uniprot
P24928 (POLR2A) S1647 Uniprot
P24928 (POLR2A) S1654 Uniprot
P24928 (POLR2A) S1668 Uniprot
P24928 (POLR2A) S1675 Uniprot
P24928 (POLR2A) S1696 Uniprot
P24928 (POLR2A) S1717 Uniprot
P24928 (POLR2A) S1724 Uniprot
P24928 (POLR2A) S1738 Uniprot
P24928 (POLR2A) S1766 Uniprot
P24928 (POLR2A) S1787 Uniprot
P24928 (POLR2A) S1864 Uniprot
P24928 (POLR2A) S1871 Uniprot
P24928 (POLR2A) S1878 Uniprot
P24928 (POLR2A) S1892 Uniprot
P24928 (POLR2A) S1899 Uniprot
P24928 (POLR2A) S1913 Uniprot
P24928 (POLR2A) S1920 Uniprot
P24928 (POLR2A) S1934 Uniprot
P24928 (POLR2A) S1944 Uniprot
P24928 (POLR2A) S1951 Uniprot
P24941 (CDK2) S46 Uniprot
P24941 (CDK2) T160 Uniprot
P24941 (CDK2) T165 Uniprot
P24941 (CDK2) Y168 Uniprot
P37231 (PPARG) S112 Uniprot
P49736 (MCM2) S4 Uniprot
P49736 (MCM2) S5 Uniprot
P49736 (MCM2) S7 Uniprot
P49736 (MCM2) S13 Uniprot
P49736 (MCM2) S27 Uniprot
P49736 (MCM2) S40 Uniprot
P49736 (MCM2) S41 Uniprot
P49736 (MCM2) S53 Uniprot
P49736 (MCM2) T59 Uniprot
P49736 (MCM2) S108 Uniprot
P49736 (MCM2) S139 Uniprot
P50613 (CDK7) S164 Uniprot
P50613 (CDK7) T170 Uniprot
Q00534 (CDK6) T177 Uniprot
Q01094 (E2F1) S403 Uniprot
Q01094 (E2F1) T433 Uniprot
Q13285 (NR5A1) S203 Uniprot
Q14847-1 (LASP1) S146 Uniprot
Q15797 (SMAD1) S206 Uniprot
Q9BYE7-1 (PCGF6) S30 Uniprot
Q9H0D6 (XRN2) T439 Uniprot
Q9NYV4 (CDK12) T893 Uniprot
Q9UPZ9 (ICK) T157 Uniprot

Research Backgrounds


Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.


Phosphorylation of Ser-164 during mitosis inactivates the enzyme. Phosphorylation of Thr-170 is required for activity. Phosphorylated at Ser-164 and Thr-170 by CDK2.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Perinuclear region.
Note: Colocalizes with PRKCI in the cytoplasm and nucleus. Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:


Subunit Structure:

Associates primarily with cyclin-H (CCNH) and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor; this complex is sensitive to UV light. The CAK complex binds to p53/TP53 in response to DNA damage. Interacts with CDK2, SF1/NR5A1, PUF60 and PRKCI.


Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Transcription > Basal transcription factors.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

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