MKRN1 Antibody - #DF13815

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Product: MKRN1 Antibody
Catalog: DF13815
Description: Rabbit polyclonal antibody to MKRN1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 55kD; 53kD(Calculated).
Uniprot: Q9UHC7

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
MKRN1 Antibody detects endogenous levels of total MKRN1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ubiquitin-protein ligase makorin-1; FLJ21334; HGNC:7112; Makorin; Makorin ring finger protein 1; MKRN 1; mkrn1; MKRN1_HUMAN; OTTHUMP00000212117; OTTHUMP00000212118; OTTHUMP00000212121; OTTHUMP00000212124; Ring finger protein 1; RING finger protein 61; RNF61;

Immunogens

Immunogen:

A synthesized peptide derived from Human MKRN1.

Uniprot:

Q9UHC7(MKRN1_HUMAN) >>Visit HPA database.

Gene(ID):
MKRN1(23608)
Expression:
Q9UHC7 MKRN1_HUMAN:

Ubiquitous.

Sequence:
MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFWELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL

Research Backgrounds

Function:

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.

PTMs:

Auto-ubiquitinated; which leads to proteasomal degradation.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with p53/TP53 and CDKN1A. Interacts with TERT, modulating telomere length homeostasis.

Restrictive clause

 

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