Interleukin 34 Antibody - #DF13820

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Product: Interleukin 34 Antibody
Catalog: DF13820
Description: Rabbit polyclonal antibody to Interleukin 34
Application: IHC IF/ICC
Cited expt.: IHC
Reactivity: Human
Mol.Wt.: 40~50kDa; 27kD(Calculated).
Uniprot: Q6ZMJ4

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Interleukin 34 Antibody detects endogenous levels of total Interleukin 34.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C16orf77; Chromosome 16 open reading frame 77; FPT025; IL 34; IL-34; Il34; IL34_HUMAN; Interleukin 34; Interleukin-34; Interleukin34; MGC34647;

Immunogens

Immunogen:

A synthesized peptide derived from Human Interleukin 34.

Uniprot:

Q6ZMJ4(IL34_HUMAN) >>Visit HPA database.

Gene(ID):
IL34(146433)
Expression:
Q6ZMJ4 IL34_HUMAN:

Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

Sequence:
MPRGFTWLRYLGIFLGVALGNEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVPSPQSCSPEPSLQYAATQLYPPPPWSPSSPPHSTGSVRPVRAQGEGLLP

Research Backgrounds

Function:

Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

Family&Domains:

Belongs to the IL-34 family.

References

1). Interactions between MFAP5 + fibroblasts and tumor-infiltrating myeloid cells shape the malignant microenvironment of colorectal cancer. Journal of translational medicine, 2023 (PubMed: 37344903) [IF=7.4]

Application: IHC    Species: human    Sample:

Fig. 6 MFAP5 + fibroblasts modulate the phenotype of C1QC + macrophages through the IL34/CSF1R signal pathway. A The hierarchy plots of CSF signaling pathway network indicate the regulation of MFAP5 + fibroblasts and FABP5 + fibroblasts on myeloid cells in normal or tumor sites. B Violin plot of IL34, CSF1, and CSF1R expression of tumor-specific fibroblasts and myeloid cells in distinct tissues (normal or tumor). C Heatmap shows dominant senders, receivers, mediators and influencers in CSF signals of tumor inferred by network centrality score. D Bar plot shows the contribution of all L-R pairs in CSF pathway within the normal tissue. E Spatial images localize the expression of IL34 and CSF1R which suggest that the co-localization of IL34 and CSF1R in CRC slices. F Heat map of M1/M2 phenotype scores of three identified tumor-associated macrophage subtypes. G Immunohistochemical double-staining of MFAP5/CD163 and IL-34/CSF1R using continuous slides of CRC tissues (×20, 50 μm)

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