Product: NAA60 Antibody
Catalog: DF13864
Description: Rabbit polyclonal antibody to NAA60
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 30kD; 27kD(Calculated).
Uniprot: Q9H7X0

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
NAA60 Antibody detects endogenous levels of total NAA60.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ11693; FLJ14154; HAT4; Histone acetyltransferase type B protein 4; N acetyltransferase 15 (GCN5 related, putative); N alpha acetyltransferase 60; N(alpha) acetyltransferase 60, NatF catalytic subunit; N-acetyltransferase 15; N-alpha-acetyltransferase 60; naa60; NAA60_HUMAN; NAT15; NatF catalytic subunit;

Immunogens

Immunogen:

A synthesized peptide derived from Human FLJ14154.

Uniprot:
Gene(ID):
Sequence:
MTEVVPSSALSEVSLRLLCHDDIDTVKHLCGDWFPIEYPDSWYRDITSNKKFFSLAATYRGAIVGMIVAEIKNRTKIHKEDGDILASNFSVDTQVAYILSLGVVKEFRKHGIGSLLLESLKDHISTTAQDHCKAIYLHVLTTNNTAINFYENRDFKQHHYLPYYYSIRGVLKDGFTYVLYINGGHPPWTILDYIQHLGSALASLSPCSIPHRVYRQAHSLLCSFLPWSGISSKSGIEYSRTM

Research Backgrounds

Function:

N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol. Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase. May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences.

PTMs:

Acetylated: autoacetylation is required for optimal acetyltransferase activity.

Subcellular Location:

Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Probably forms an intramembrane hairpin-like structure in the membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer and homodimer; monomer in presence of substrate and homodimer in its absence.

Family&Domains:

Belongs to the acetyltransferase family. NAA60 subfamily.

Restrictive clause

 

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