Product: Synapsin I Antibody
Catalog: AF6201
Description: Rabbit polyclonal antibody to Synapsin I
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Xenopus
Mol.Wt.: 77kDa; 74kD(Calculated).
Uniprot: P17600
RRID: AB_2835082

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Synapsin I Antibody detects endogenous levels of total Synapsin I.
RRID:
AB_2835082
Cite Format: Affinity Biosciences Cat# AF6201, RRID:AB_2835082.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Brain protein 4.1; SYN 1; SYN 1a; SYN 1b; SYN I; SYN1; SYN1_HUMAN; SYN1a; SYN1b; Synapsin 1; Synapsin I; Synapsin-1; Synapsin1; SynapsinI; SYNI;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases.
Sequence:
MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPGAHSPGATPGPGTATAERSSGVAPAASPAAPSPGSSGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAASRVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVKVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMAQALPRQRQRDASPGRGSHGQTPSPGALPLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPAGSPLPQRLPSPTSAPQQPASQAAPPTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGPPQATRQTSVSGPAPPKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPVPRTGPPTTQQPRPSGPGPAGRPKPQLAQKPSQDVPPPATAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFASLFSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Rabbit
100
Horse
0
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17600 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation Q14012 (CAMK1) , Q13131 (PRKAA1)
S11 Phosphorylation
S39 Phosphorylation
S62 Phosphorylation P27361 (MAPK3)
S67 Phosphorylation P27361 (MAPK3)
S293 Phosphorylation
Y301 Phosphorylation
S332 Phosphorylation
S427 Phosphorylation
S432 Phosphorylation
T436 Phosphorylation
S438 Phosphorylation Q8TAS1 (UHMK1)
T448 Phosphorylation
S449 Phosphorylation
S551 Phosphorylation P27361 (MAPK3) , Q00535 (CDK5)
S553 Phosphorylation Q00535 (CDK5)
T567 Phosphorylation
S568 Phosphorylation
S605 Phosphorylation
T613 Phosphorylation
S698 Phosphorylation

Research Backgrounds

Function:

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.

PTMs:

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal.

Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.

Subcellular Location:

Cell junction>Synapse. Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ib interacts with PRNP (By similarity).

Family&Domains:

The A region binds phospholipids with a preference for negatively charged species.

Belongs to the synapsin family.

References

1). Shen C et al. SiNiSan ameliorates depression-like behavior in rats by enhancing synaptic plasticity via the CaSR-PKC-ERK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY 2020 Jan 17;124:109787 (PubMed: 31958763) [IF=7.5]

Application: WB    Species: rat    Sample: HIP

Fig. 5. |Effects of SNS on synaptic-associated protein in the HIP and PFC of stressed rats. Representative immunoblots for PSD-95, GAP-43, Syn, and Tublin in the HIP(A) and PFC(B) regions. (A) Results of relative protein levels of PSD-95, GAP-43, and Syn in the HIP of rats of each group.

2). Li WY et al. Exosomes derived from human induced pluripotent stem cell-derived neural progenitor cells protect neuronal function under ischemic conditions. Neural Regeneration Research 2021 Oct;16(10):2064-2070. (PubMed: 33642395) [IF=6.1]

Application: WB    Species: Rat    Sample: neural progenitor cells

Figure 3 Effect of iPSC-NPC-derived exosomes (OGD+iNPC-exo) on expression of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins in OGD induced neurons. (A–C) mRNA expression (A) and protein expression (B, C) of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins (NF200, GAP-43, Synapsin, and PSD95) analyzed by polymerase chain reaction and western blot assay. The mRNA expression is described by the optical density ratio relative to the control group. Protein expression was described by the optical density ratio relative to β-actin. Data are presented as mean ± SD. ***P < 0.001, vs. control group; ###P < 0.001 (one-way analysis of variance with post hoc Bonferroni test). All experiments were repeated three times. GAP43: growth associated protein 43; iPSC-NPCs: induced pluripotent stem cells-derived neural progenitor cells; NF200: neurofilament 200; OGD: oxygen-glucose deprivation; p-Akt: phosphor-Akt; PSD95: postsynaptic density protein 95; PTEN: phosphatase and tensin homolog deleted on chromosome ten.

3). Cui Y et al. Early-Life Stress Induces Depression-Like Behavior and Synaptic-Plasticity Changes in a Maternal Separation Rat Model: Gender Difference and Metabolomics Study. Frontiers in Pharmacology 2020;11:102 (PubMed: 32174832) [IF=5.6]

Application: WB    Species: Rat    Sample: hippocampus

Figure 5 MS reduces the expression of synaptic-plasticity protein. (A) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in the hippocampus by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. (B) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in cortex by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. Statistical analyses are performed by two-way ANOVA followed by t-test. Data are presented as mean ± SEM, *p < 0.05, **p < 0.01, n = 3 per group.

4). Huang J et al. The Effect of Early Maternal Separation Combined With Adolescent Chronic Unpredictable Mild Stress on Behavior and Synaptic Plasticity in Adult Female Rats. Frontiers in Psychiatry 2021 Mar 5;12:539299. (PubMed: 33746787) [IF=4.7]

Application: WB    Species: Rat    Sample: Hippocampus

Figure 5 Western blot analysis. The expressions of synaptic plasticity proteins were determined by western blot (A), including PSD-95 (B), GAP-43 (C), and SYN (D). The values represent the mean ± SEM, n = 5. *p < 0.05, **p < 0.01 vs. CON, #p < 0.05, ##p < 0.01 vs. MS. CON, control; MS, maternal separation; CUMS, chronic unpredictable mild stress.

5). Zhang Q et al. Adverse effects of iron deficiency anemia on pregnancy outcome and offspring development and intervention of three iron supplements. Scientific Reports 2021 Jan 14;11(1):1347. (PubMed: 33446747) [IF=4.6]

Application: WB    Species: Rat    Sample: hippocampus

Figure 5 Iron related indexes and neural development of offspring rats after iron supplement treatment. Western blot analysis for FTL and Tf in liver (a–c) and spleen (d–f). Morris water maze test for day 1 escape latency (g) and day 2 escape latency (h). Western blot analysis for FTL and Tf in brain (i–k) and hippocampus (l–n), and for SYN1, NMDAR, PSD-95 in hippocampus (o–r). The quantification of western blotting was provided in supplementary material. FTL ferritin light chain, Tf transferrin, SYN1 synapsin 1, NMDAR N-methyl-D-aspartate receptor, PSD-95 postsynaptic density protein 95. Data of Western blot analysis (mean ± SD) are expressed as the ratio of the relative contents between the value from IDA group and NG group and six iron treatment groups (n = 3). The relative contents of target proteins were quantified using the ratio between the optical density (OD) of target protein and the amount of the housekeeping protein GAPDH. **p < 0.01, compared with NG, #p < 0.05, ##p < 0.01, compared with IDAG. One-way ANOVA followed by Tukey multiple comparison test was used for comparison among 8 different groups.

6). Pang RQ et al. Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer\'s Disease Model Mice. JOURNAL OF ALZHEIMERS DISEASE 2019 Sep 18 (PubMed: 31561359) [IF=4.0]

Application: WB    Species: mouse    Sample: hippocampus

Fig. 4. The expression of PSD-95, SYN, and BDNF. Representative immunoblots of PSD-95, SYN, and BDNF in the cortex (A-D) and hippocampus (E-H) in each group. Protein immunoreactivity was normalized to -actin. Individual data are presented as the mean ± S.E.M. from 4 individual mice in each group.

7). Ye R et al. Stress causes cognitive impairment by affecting cholesterol efflux and reuptake leading to abnormalities in lipid metabolism of rats. Journal of integrative neuroscience 2020 Mar 30;19(1):39-49 (PubMed: 32259885)

Application: WB    Species: rat    Sample: hippocampus

Figure 4. |Tau protein phosphorylation and impairment of synaptic plasticity in CUMS rats. (A) The expression of p-Tau and Tau in control rats and CUMS rats in the sixth week and tenth week of the CUMS period.(B) The expression of PSD-95 and SYN in control rats and CUMS rats in the sixth week and tenth week of the CUMS period. Bars represent mean ± S.E.M of the different groups (n = 3/group). (compared with control rats, *P < 0.05; **P < 0.01).

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