THEM4 Antibody | Affinity Biosciences

Product:	THEM4 Antibody
Catalog:	DF13871
Description:	Rabbit polyclonal antibody to THEM4
Application:	ELISA(peptide)
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	27kD; 27kD(Calculated).
Uniprot:	Q5T1C6

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

THEM4 Antibody detects endogenous levels of total THEM4.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acyl CoA thioesterase THEM4; Acyl coenzyme A thioesterase THEM4; C terminal modulator protein; Carboxyl-terminal modulator protein; CTMP; FLJ27206; MGC29636; OTTHUMP00000015248; Them4; THEM4_HUMAN; Thioesterase superfamily member 4;

Immunogens

Immunogen:

A synthesized peptide derived from Human THEM4.

Uniprot:

Q5T1C6(THEM4_HUMAN) >>Visit HPA database.

Gene(ID):

THEM4(117145)

Expression:

Q5T1C6 THEM4_HUMAN:

Expressed predominantly in skeletal muscle, testis, uterus, brain and kidney. Down-regulated in glioblastoma or glioma compared to non-neoplastic brain due to promoter hypermethylation.

Sequence:

Q5T1C6 THEM4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLRSCAARLRTLGALCLPPVGRRLPGSEPRPELRSFSSEEVILKDCSVPNPSWNKDLRLLFDQFMKKCEDGSWKRLPSYKRTPTEWIQDFKTHFLDPKLMKEEQMSQAQLFTRSFDDGLGFEYVMFYNDIEKRMVCLFQGGPYLEGPPGFIHGGAIATMIDATVGMCAMMAGGIVMTANLNINYKRPIPLCSVVMINSQLDKVEGRKFFVSCNVQSVDEKTLYSEATSLFIKLNPAKSLT

Research Backgrounds

Q5T1C6_THEM4_HUMAN

Function:

Has acyl-CoA thioesterase activity towards medium and long-chain (C14 to C18) fatty acyl-CoA substrates, and probably plays a role in mitochondrial fatty acid metabolism. Plays a role in the apoptotic process, possibly via its regulation of AKT1 activity. According to inhibits AKT1 phosphorylation and activity. According to enhances AKT1 activity by favoring its phosphorylation and translocation to plasma membrane.

PTMs:

Phosphorylated.

Subcellular Location:

Cell membrane. Cell projection>Ruffle membrane. Cytoplasm. Mitochondrion. Mitochondrion inner membrane>Peripheral membrane protein. Mitochondrion intermembrane space.
Note: Released from the mitochondria into the cytosol in response to apoptotic stimuli.

Tissue Specificity:

Expressed predominantly in skeletal muscle, testis, uterus, brain and kidney. Down-regulated in glioblastoma or glioma compared to non-neoplastic brain due to promoter hypermethylation.

Subunit Structure:

Homodimer and homotetramer. Interacts with AKT1 in the cytosol.

Family&Domains:

Belongs to the THEM4/THEM5 thioesterase family.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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THEM4 Antibody - #DF13871