RSPH4A Antibody - #DF13889

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Product: RSPH4A Antibody
Catalog: DF13889
Description: Rabbit polyclonal antibody to RSPH4A
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Rat
Mol.Wt.: 81kD; 81kD(Calculated).
Uniprot: Q5TD94

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
RSPH4A Antibody detects endogenous levels of total RSPH4A.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A230081C05; CILD11; dJ412I7.1; Radial spoke head protein 4 homolog A; Radial spoke head-like protein 3; RSH4A_HUMAN; RSHL3; Rsph4a; RSPH6B;

Immunogens

Immunogen:

A synthesized peptide derived from Human RSPH4A.

Uniprot:

Q5TD94(RSH4A_HUMAN) >>Visit HPA database.

Gene(ID):
RSPH4A(345895)
Expression:
Q5TD94 RSH4A_HUMAN:

Expressed in trachea, lungs, and testes. Very strong expression is detected in nasal brushings.

Sequence:
MEDSTSPKQEKENQEELGETRRPWEGKTAASPQYSEPESSEPLEAKQGPETGRQSRSSRPWSPQSRAKTPLGGPAGPETSSPAPVSPREPSSSPSPLAPARQDLAAPPQSDRTTSVIPEAGTPYPDPLEQSSDKRESTPHHTSQSEGNTFQQSQQPKPHLCGRRDVSYNNAKQKELRFDVFQEEDSNSDYDLQQPAPGGSEVAPSMLEITIQNAKAYLLKTSSNSGFNLYDHLSNMLTKILNERPENAVDIFENISQDVKMAHFSKKFDALQNENELLPTYEIAEKQKALFLQGHLEGVDQELEDEIAENALPNVMESAFYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFREGEDEEEVEEEDVAEERDNGESEAHEDEEDELPKSFYKAPQAIPKEESRTGANKYVYFVCNEPGRPWVKLPPVIPAQIVIARKIKKFFTGRLDAPIISYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEEAEGGRNSFEENPDFEGIQVIDLVESLSNWVHHVQHILSQGRCNWFNSIQKNEEEEEEEDEEKDDSDYIEQEVGLPLLTPISEDLEIQNIPPWTTRLSSNLIPQYAIAVLQSNLWPGAYAFSNGKKFENFYIGWGHKYSPDNYTPPVPPPVYQEYPSGPEITEMDDPSVEEEQAFRAAQEAVLLAAENEESEEDEDEEDDYD

Research Backgrounds

Function:

Probable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules.

Subcellular Location:

Cytoplasm>Cytoskeleton>Cilium axoneme. Cell projection>Cilium.
Note: Radial spoke.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in trachea, lungs, and testes. Very strong expression is detected in nasal brushings.

Family&Domains:

Belongs to the flagellar radial spoke RSP4/6 family.

References

1). Association of novel DNAH11 variants with asthenoteratozoospermia lead to male infertility. Human genomics, 2024 (PubMed: 39256880) [IF=3.8]

Application: WB    Species: human    Sample:

Fig. 5 The distribution and expression levels of flagella-associated proteins in patients. (A-D). Immunofluorescence staining assays were performed on the sperm of mutant patients and normal subjects using anti-DNAI2 (red), anti-SPAG6 (red), anti-RSPH4A (red), and anti-TOMM20 (red). The results showed that TOMM20 was located at the mitochondrial sheath, and RSPH4A, DNAI2, and SPAG6 were distributed in full-length flagella in fertile control individual sperm. By contrast, it was found that almost absence of DNAI2, SPAG6, and TOMM20 in sperm from AY0524 and AY0540, RSPH4A was accumulated on the sperm neck, while it was not evidently changed in DNAH9-deficient sperm. Anti-ac-tubulin (green) marked the sperm flagella, and Hoechst (blue) marked the nucleus of spermatozoa. Scale bars: 10 μm. (E) The levels of DNAI2, SPAG6, RSPH4A, and TOMM20 in sperm of patients (AY0524, AY0540, XFY03, and XFY04) reduced markedly compared with control individuals by western blotting analysis. The results of WB assays were in accordance with those of immunofluorescence assays described above. β-actin was used as an internal reference

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