ATOX1 Antibody - #DF13934

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Product: ATOX1 Antibody
Catalog: DF13934
Description: Rabbit polyclonal antibody to ATOX1
Application: IHC IF/ICC
Cited expt.: IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 10kD; 7kD(Calculated).
Uniprot: O00244

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
ATOX1 Antibody detects endogenous levels of total ATOX1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATOX1; ATOX1_HUMAN; ATX1; ATX1 antioxidant protein 1 homolog (yeast); ATX1 antioxidant protein 1 homolog; Copper transport protein; Copper transport protein ATOX1; HAH1; Metal transport protein; Metal transport protein ATX1; MGC138453; MGC138455;

Immunogens

Immunogen:

A synthesized peptide derived from Human ATOX1.

Uniprot:

O00244(ATOX1_HUMAN) >>Visit HPA database.

Gene(ID):
ATOX1(475)
Expression:
O00244 ATOX1_HUMAN:

Ubiquitous.

Sequence:
MPKHEFSVDMTCGGCAEAVSRVLNKLGGVKYDIDLPNKKVCIESEHSMDTLLATLKKTGKTVSYLGLE

Research Backgrounds

Function:

Binds and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.

Tissue Specificity:

Ubiquitous.

Family&Domains:

Belongs to the ATX1 family.

Research Fields

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Curcumin suppresses copper accumulation in non-small cell lung cancer by binding ATOX1. BMC pharmacology & toxicology, 2024 (PubMed: 39169392) [IF=2.9]

Application: WB    Species: human    Sample: A549 and H1299 cells

Fig. 1 Effects of curcumin on ATOX1-associated copper chaperones in A549 and H1299 cells. (A) The binding activity between curcumin and ATOX1 protein was analyzed using molecular docking. (B) Cell viability was measured by CCK-8. (C) Levels of ATOX1, ATP7A, and COX17 proteins were measured by western blotting. The levels of proteins were normalized by β-actin. The full-length blots were shown in Supplementary Fig. 1 and Fig. 2. (D) The semi-quantitative analysis of the blots was analyzed by ImageJ software. (E) Effects of DC-AC50 treatment on cell viability. Three parallel experiments were performed in cells. NS no significance. *p 

Application: IHC    Species: human    Sample: A549 cells

Fig. 5 Curcumin inhibited tumor growth by suppressing ATOX1. The animal model was established by injection of A549 cells. After 6 days of injection, the mice were injected with 20 mg/kg/d DC-AC50 or 40 mg/kg/d curcumin by intraperitoneal injection for 21 days. (A) Tumor volume was measured every week. (B) Tumor weight was obtained. Expressions of ATOX1 in tumor tissues were measured by immunohistochemistry (C) and western blotting (D). Black arrows: ATOX1 expression. The full-length blots were presented in Supplementary Fig. 7. Six animals in each group. *p 

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For Research Use Only.
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