PARL Antibody | Affinity Biosciences

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PARL Antibody - DF14001 at 1/100 staining mouse brain tissue by IHC-P.

Product:	PARL Antibody
Catalog:	DF14001
Description:	Rabbit polyclonal antibody to PARL
Application:	IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	42kD; 42kD(Calculated).
Uniprot:	Q9H300

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

PARL Antibody detects endogenous levels of total PARL.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Mitochondrial intramembrane cleaving protease PARL; Mitochondrial intramembrane-cleaving protease PARL; P-beta; parl; PARL_HUMAN; Pbeta; presenilin associated rhomboid-like; Presenilins associated rhomboid-like protein; PRO2207; PSARL; PSARL1; PSENIP2; RHBDS1; Rhomboid 7 homolog 1;

Immunogens

Immunogen:

A synthesized peptide derived from Human PARL.

Uniprot:

Q9H300(PARL_HUMAN) >>Visit HPA database.

Gene(ID):

PARL(55486)

Sequence:

Q9H300 PARL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAWRGWAQRGWGCGQAWGASVGGRSCEELTAVLTPPQLLGRRFNFFIQQKCGFRKAPRKVEPRRSDPGTSGEAYKRSALIPPVEETVFYPSPYPIRSLIKPLFFTVGFTGCAFGSAAIWQYESLKSRVQSYFDGIKADWLDSIRPQKEGDFRKEINKWWNNLSDGQRTVTGIIAANVLVFCLWRVPSLQRTMIRYFTSNPASKVLCSPMLLSTFSHFSLFHMAANMYVLWSFSSSIVNILGQEQFMAVYLSAGVISNFVSYVGKVATGRYGPSLGASGAIMTVLAAVCTKIPEGRLAIIFLPMFTFTAGNALKAIIAMDTAGMILGWKFFDHAAHLGGALFGIWYVTYGHELIWKNREPLVKIWHEIRTNGPKKGGGSK

Research Backgrounds

Q9H300_PARL_HUMAN

Function:

Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals (By similarity). Required for the maturation of PINK1 into its 52kDa mature form after its cleavage by mitochondrial-processing peptidase (MPP). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain.

PTMs:

P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner. The cleavage is inhibited when residues Ser-65, Thr-69 and Ser-70 are all phosphorylated.

Subcellular Location:

Mitochondrion inner membrane>Multi-pass membrane protein.

Nucleus.
Note: Translocated into the nucleus by an unknown mechanism (PubMed:17116872).

Subunit Structure:

Interacts with PSEN1 and PSEN2. Binds OPA1.

Family&Domains:

Belongs to the peptidase S54 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PARL Antibody - #DF14001