Product: SOX15 Antibody
Catalog: DF14017
Description: Rabbit polyclonal antibody to SOX15
Application: WB IHC
Reactivity: Human
Mol.Wt.: 25kD; 25kD(Calculated).
Uniprot: O60248

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
SOX15 Antibody detects endogenous levels of total SOX15.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Protein SOX-12; Protein SOX-15; Protein SOX-20; SOX 12 protein; SOX 15; SOX 15 protein; SOX 20 protein; SOX12; SOX12 protein; SOX15; SOX15 protein; SOX15_HUMAN; SOX20; SOX20 protein; SOX26; SOX27; SRY box 15; SRY sex determining region Y box 15; SRY sex determining region Y box 20; SRYbox 15;

Immunogens

Immunogen:

A synthesized peptide derived from Human SOX15.

Uniprot:
Gene(ID):
Expression:
O60248 SOX15_HUMAN:

Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Sequence:
MALPGSSQDQAWSLEPPAATAAASSSSGPQEREGAGSPAAPGTLPLEKVKRPMNAFMVWSSAQRRQMAQQNPKMHNSEISKRLGAQWKLLDEDEKRPFVEEAKRLRARHLRDYPDYKYRPRRKAKSSGAGPSRCGQGRGNLASGGPLWGPGYATTQPSRGFGYRPPSYSTAYLPGSYGSSHCKLEAPSPCSLPQSDPRLQGELLPTYTHYLPPGSPTPYNPPLAGAPMPLTHL

Research Backgrounds

Function:

Transcription factor that binds to DNA at the 5'-AACAATG-3' consensus sequence (By similarity). Acts as a transcriptional activator and repressor (By similarity). Binds synergistically with POU5F1 (OCT3/4) to gene promoters (By similarity). Binds to the FOXK1 promoter and recruits FHL3, resulting in transcriptional activation of FOXK1 which leads to myoblast proliferation (By similarity). Acts as an inhibitor of myoblast differentiation via transcriptional repression which leads to down-regulation of the muscle-specific genes MYOD and MYOG (By similarity). Involved in trophoblast giant cell differentiation via enhancement of HAND1 transcriptional activity (By similarity). Regulates transcription of HRC via binding to it proximal enhancer region (By similarity). Involved in skeletal muscle regeneration (By similarity). Also plays a role in the development of myogenic precursor cells (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Subunit Structure:

Interacts with HAND1; the interaction enhances HAND1-induced differentiation of trophoblast giant cells (By similarity). Interacts with POU5F1 (OCT3/4); binds synergistically with POU5F1 to DNA (By similarity). Interacts with FHL3; the interaction recruits the transcriptional coactivator FHL3 to the FOXK1 promoter (By similarity).

Restrictive clause

 

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For Research Use Only.
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