SLC5A4 Antibody | Affinity Biosciences

IF/ICC

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SLC5A4 Antibody - DF14033 staining Hela cells by IF/ICC.

Product:	SLC5A4 Antibody
Catalog:	DF14033
Description:	Rabbit polyclonal antibody to SLC5A4
Application:	IF/ICC
Reactivity:	Human
Mol.Wt.:	72kD; 72kD(Calculated).
Uniprot:	Q9NY91

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

SLC5A4 Antibody detects endogenous levels of total SLC5A4.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DJ90G24.4; Low affinity sodium glucose cotransporter; Na(+)/glucose cotransporter 3; SAAT1; SGLT2; SGLT3; Sodium/glucose cotransporter 3; Solute carrier family 5 member 4;

Immunogens

Immunogen:

A synthesized peptide derived from Human SLC5A4.

Uniprot:

Q9NY91(SC5A4_HUMAN) >>Visit HPA database.

Gene(ID):

SLC5A4(6527)

Expression:

Q9NY91 SC5A4_HUMAN:

Expressed in skeletal muscle, where it may localize to the neuromuscular junction (at protein level) (PubMed:13130073). Expressed in small intestine where it may localize to cholinergic neurons of the submucosal plexus and myenteric plexus (at protein level) (PubMed:13130073). Detected in kidney (at protein level) (PubMed:22766068).

Sequence:

Q9NY91 SC5A4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASTVSPSTIAETPEPPPLSDHIRNAADISVIVIYFLVVMAVGLWAMLKTNRGTIGGFFLAGRDMAWWPMGASLFASNIGSNHYVGLAGTGAASGVATVTFEWTSSVMLLILGWIFVPIYIKSGVMTMPEYLKKRFGGERLQVYLSILSLFICVVLLISADIFAGAIFIKLALGLDLYLAIFILLAMTAVYTTTGGLASVIYTDTLQTIIMLIGSFILMGFAFNEVGGYESFTEKYVNATPSVVEGDNLTISASCYTPRADSFHIFRDAVTGDIPWPGIIFGMPITALWYWCTNQVIVQRCLCGKDMSHVKAACIMCAYLKLLPMFLMVMPGMISRILYTDMVACVVPSECVKHCGVDVGCTNYAYPTMVLELMPQGLRGLMLSVMLASLMSSLTSIFNSASTLFTIDLYTKMRKQASEKELLIAGRIFVLLLTVVSIVWVPLVQVSQNGQLIHYTESISSYLGPPIAAVFVLAIFCKRVNEQGAFWGLMVGLAMGLIRMITEFAYGTGSCLAPSNCPKIICGVHYLYFSIVLFFGSMLVTLGISLLTKPIPDVHLYRLCWVLRNSTEERIDIDAEEKSQEETDDGVEEDYPEKSRGCLKKAYDLFCGLQKGPKLTKEEEEALSKKLTDTSERPSWRTIVNINAILLLAVVVFIHGYYA

Research Backgrounds

Q9NY91_SC5A4_HUMAN

Function:

Has electrogenic activity in response to glucose, and may function as a glucose sensor. Mediates influx of sodium ions into the cell but does not transport sugars. Also potently activated by imino sugars such as deoxynojirimycin (DNJ).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed in skeletal muscle, where it may localize to the neuromuscular junction (at protein level). Expressed in small intestine where it may localize to cholinergic neurons of the submucosal plexus and myenteric plexus (at protein level). Detected in kidney (at protein level).

Family&Domains:

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SLC5A4 Antibody - #DF14033