RAD51AP1 Antibody - #DF14175

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Product: RAD51AP1 Antibody
Catalog: DF14175
Description: Rabbit polyclonal antibody to RAD51AP1
Application: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 40kD; 38kD(Calculated).
Uniprot: Q96B01

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
RAD51AP1 Antibody detects endogenous levels of total RAD51AP1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2510006L10Rik; MGC156698; OTTMUSP00000028651; PIR51; R51A1_HUMAN; RAB22; RAD51 associated protein 1; RAD51 interacting protein; RAD51-associated protein 1; RAD51-interacting protein; Rad51ap1;

Immunogens

Immunogen:

A synthesized peptide derived from Human RAD51AP1.

Uniprot:

Q96B01(R51A1_HUMAN) >>Visit HPA database.

Gene(ID):
RAD51AP1(10635)
Expression:
Q96B01 R51A1_HUMAN:

Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.

Sequence:
MVRPVRHKKPVNYSQFDHSDSDDDFVSATVPLNKKSRTAPKELKQDKPKPNLNNLRKEEIPVQEKTPKKRLPEGTFSIPASAVPCTKMALDDKLYQRDLEVALALSVKELPTVTTNVQNSQDKSIEKHGSSKIETMNKSPHISNCSVASDYLDLDKITVEDDVGGVQGKRKAASKAAAQQRKILLEGSDGDSANDTEPDFAPGEDSEDDSDFCESEDNDEDFSMRKSKVKEIKKKEVKVKSPVEKKEKKSKSKCNALVTSVDSAPAAVKSESQSLPKKVSLSSDTTRKPLEIRSPSAESKKPKWVPPAASGGSRSSSSPLVVVSVKSPNQSLRLGLSRLARVKPLHPNATST

Research Backgrounds

Function:

May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.

Subcellular Location:

Nucleus.
Note: Colocalizes with RAD51 to multiple nuclear foci.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.

Subunit Structure:

Isoform 2 interacts with RAD51. Interacts with PALB2. Interacts (via C-terminal region) with RAD51; the interaction is direct.

Restrictive clause

 

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