Product: SNX33 Antibody
Catalog: DF14186
Description: Rabbit polyclonal antibody to SNX33
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 65kD(Calculated).
Uniprot: Q8WV41

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
SNX33 Antibody detects endogenous levels of total SNX33.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC32065; SH3 and PX domain containing 3; SH3 and PX domain containing protein 3; SH3 and PX domain-containing protein 3; SH3PX3; SH3PXD3C; SNX 30; SNX 33; SNX30; snx33; SNX33_HUMAN; Sorting nexin 33; Sorting nexin-33;

Immunogens

Immunogen:

A synthesized peptide derived from Human SNX33.

Uniprot:
Gene(ID):
Expression:
Q8WV41 SNX33_HUMAN:

Detected in heart and pancreas.

Sequence:
MALKGRALYDFHSENKEEISIQQDEDLVIFSETSLDGWLQGQNSRGETGLFPASYVEIVRSGISTNHADYSSSPAGSPGAQVSLYNSPSVASPARSGGGSGFLSNQGSFEEDDDDDWDDWDDGCTVVEEPRAGGLGTNGHPPLNLSYPGAYPSQHMAFRPKPPLERQDSLASAKRGSVVGRNLNRFSCFVRSGVEAFILGDVPMMAKIAETYSIEMGPRGPQWKANPHPFACSVEDPTKQTKFKGIKSYISYKLTPTHAASPVYRRYKHFDWLYNRLLHKFTVISVPHLPEKQATGRFEEDFIEKRKRRLILWMDHMTSHPVLSQYEGFQHFLSCLDDKQWKMGKRRAEKDEMVGASFLLTFQIPTEHQDLQDVEDRVDTFKAFSKKMDDSVLQLSTVASELVRKHVGGFRKEFQKLGSAFQAISHSFQMDPPFCSEALNSAISHTGRTYEAIGEMFAEQPKNDLFQMLDTLSLYQGLLSNFPDIIHLQKGAFAKVKESQRMSDEGRMVQDEADGIRRRCRVVGFALQAEMNHFHQRRELDFKHMMQNYLRQQILFYQRVGQQLEKTLRMYDNL

Research Backgrounds

Function:

Plays a role in the reorganization of the cytoskeleton, endocytosis and cellular vesicle trafficking via its interactions with membranes, WASL, DNM1 and DNM2. Acts both during interphase and at the end of mitotic cell divisions. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Modulates endocytosis of cell-surface proteins, such as APP and PRNP; this then modulates the secretion of APP and PRNP peptides. Promotes membrane tubulation (in vitro). May promote the formation of macropinosomes.

PTMs:

Phosphorylated.

Subcellular Location:

Cytoplasm>Cytosol. Membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Primarily cytosolic, but a minor proportion is membrane-bound (PubMed:18353773). Not associated with membranes (PubMed:21048941).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in heart and pancreas.

Subunit Structure:

Homodimer (via BAR domain). Interacts with ADAM15. Interacts with FASLG. Interacts (via SH3 domain) with DNM1 and DNM2. Interacts with WASL. Interacts with FCHSD1 (via the F-BAR domain) (By similarity).

Family&Domains:

The PX and BAR domains mediate association with membranes and are required for membrane tubulation.

Belongs to the sorting nexin family.

Restrictive clause

 

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