Product: RHOJ Antibody
Catalog: DF14204
Description: Rabbit polyclonal antibody to RHOJ
Application: IHC
Reactivity: Human
Mol.Wt.: 24kD; 24kD(Calculated).
Uniprot: Q9H4E5

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
RHOJ Antibody detects endogenous levels of total RHOJ.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHJ; Ras homolog family member J; Ras homolog gene family member J; RAS like family 7 member B; Ras like protein family member 7B; Ras-like protein family member 7B; RASL 7B; RASL7B; Rho related GTP binding protein RhoJ; Rho-related GTP-binding protein RhoJ; RHOI; Rhoj; RHOJ_HUMAN; Tc10 like GTP binding protein TCL; TC10 like Rho GTPase; Tc10-like GTP-binding protein; TC10B; TCL;

Immunogens

Immunogen:

A synthesized peptide derived from Human RHOJ.

Uniprot:
Gene(ID):
Expression:
Q9H4E5 RHOJ_HUMAN:

Specifically expressed in endothelial cells in different tissues, such as brain, heart, lung and liver.

Sequence:
MNCKEGTDSSCGCRGNDEKKMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHLLGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKRCSEGHSCCSII

Research Backgrounds

Function:

Plasma membrane-associated small GTPase specifically involved in angiogenesis. Required for endothelial cell migration during vascular development via its interaction with GLUL. Elicits the formation of F-actin-rich structures, thereby regulating endothelial cell migration.

PTMs:

Palmitoylated; regulates localization to the plasma membrane and may be mediated by GLUL.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.
Note: Localization to the plasma membrane is regulated by GLUL.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Specifically expressed in endothelial cells in different tissues, such as brain, heart, lung and liver.

Subunit Structure:

Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp (By similarity). Interacts with GLUL.

Family&Domains:

Belongs to the small GTPase superfamily. Rho family.

Restrictive clause

 

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