Product: CD130/gp130 Antibody
Catalog: AF6291
Description: Rabbit polyclonal antibody to CD130/gp130
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 160kDa; 104kD(Calculated).
Uniprot: P40189
RRID: AB_2835141

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
CD130/gp130 Antibody detects endogenous levels of total CD130/gp130.
RRID:
AB_2835141
Cite Format: Affinity Biosciences Cat# AF6291, RRID:AB_2835141.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD130; CD130 antigen; CDw130; gp130; GP130 RAPS; IL 6R beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; IL6 ST; IL6RB_HUMAN; IL6ST; Interleukin 6 receptor subunit beta; Interleukin receptor beta chain; Interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; Membrane glycoprotein gp130; Oncostatin M receptor; Oncostatin M receptor alpha subunit; Oncostatin-M receptor subunit alpha;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P40189 IL6RB_HUMAN:

Found in all the tissues and cell lines examined (PubMed:2261637). Expression not restricted to IL6 responsive cells (PubMed:2261637). Expressed in blood serum (at protein level) (PubMed:24629561).

Description:
The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors.
Sequence:
MLTLQTWLVQALFIFLTTESTGELLDPCGYISPESPVVQLHSNFTAVCVLKEKCMDYFHVNANYIVWKTNHFTIPKEQYTIINRTASSVTFTDIASLNIQLTCNILTFGQLEQNVYGITIISGLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVTLTRWKSHLQNYTVNATKLTVNLTNDRYLATLTVRNLVGKSDAAVLTIPACDFQATHPVMDLKAFPKDNMLWVEWTTPRESVKKYILEWCVLSDKAPCITDWQQEDGTVHRTYLRGNLAESKCYLITVTPVYADGPGSPESIKAYLKQAPPSKGPTVRTKKVGKNEAVLEWDQLPVDVQNGFIRNYTIFYRTIIGNETAVNVDSSHTEYTLSSLTSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIEAIVVPVCLAFLLTTLLGVLFCFNKRDLIKKHIWPNVPDPSKSHIAQWSPHTPPRHNFNSKDQMYSDGNFTDVSVVEIEANDKKPFPEDLKSLDLFKKEKINTEGHSSGIGGSSCMSSSRPSISSSDENESSQNTSSTVQYSTVVHSGYRHQVPSVQVFSRSESTQPLLDSEERPEDLQLVDHVDGGDGILPRQQYFKQNCSQHESSPDISHFERSKQVSSVNEEDFVRLKQQISDHISQSCGSGQMKMFQEVSAADAFGPGTEGQVERFETVGMEAATDEGMPKSYLPQTVRQGGYMPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
86
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40189 As Substrate

Site PTM Type Enzyme
N43 N-Glycosylation
N83 N-Glycosylation
N131 N-Glycosylation
K140 Ubiquitination
N157 N-Glycosylation
K168 Ubiquitination
N227 N-Glycosylation
Y309 Phosphorylation
Y322 Phosphorylation
N379 N-Glycosylation
N383 N-Glycosylation
N390 N-Glycosylation
Y396 Phosphorylation
N553 N-Glycosylation
N564 N-Glycosylation
K649 Ubiquitination
S661 Phosphorylation
S667 Phosphorylation
T670 Phosphorylation
S678 Phosphorylation
Y683 Phosphorylation
K702 Ubiquitination
S710 Phosphorylation
K715 Ubiquitination
K718 Ubiquitination
Y759 Phosphorylation
T761 Phosphorylation
Y767 Phosphorylation
S780 Phosphorylation
S782 Phosphorylation
T783 Phosphorylation
Y814 Phosphorylation
S820 Phosphorylation
S824 Phosphorylation
S825 Phosphorylation
S829 Phosphorylation
K835 Ubiquitination
S838 Phosphorylation
S839 Phosphorylation
K849 Methylation
K849 Ubiquitination
S853 Phosphorylation
T890 Phosphorylation Q05655 (PRKCD)
T897 Phosphorylation
Y905 Phosphorylation
T909 Phosphorylation Q05655 (PRKCD)
Y915 Phosphorylation

PTMs - P40189 As Enzyme

Substrate Site Source
O60674 (JAK2) Y1007 Uniprot
O60674 (JAK2) Y1008 Uniprot

Research Backgrounds

Function:

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates Janus kinases. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (By similarity). Has a role in embryonic development (By similarity). Does not bind IL6. Essential for survival of motor and sensory neurons and for differentiation of astrocytes (By similarity). Required for expression of TRPA1 in nociceptive neurons (By similarity). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (By similarity). Required for normal trabecular bone mass and cortical bone composition (By similarity).

PTMs:

Phosphorylation of Ser-782 down-regulates cell surface expression.

Heavily N-glycosylated. Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. Expressed in blood serum (at protein level).

Subunit Structure:

Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Forms heterodimers composed of LIFR and IL6ST (type I OSM receptor) which are activated by LIF and OSM. Also forms heterodimers composed of OSMR and IL6ST (type II receptor) which are activated by OSM but not by LIF. Homodimer. Interacts with HCK. Interacts with INPP5D/SHIP1 (By similarity).

(Microbial infection) The homodimer binds two molecules of herpes virus 8/HHV-8 protein vIL-6.

Family&Domains:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 2 subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

References

1). Li X et al. Antinociceptive effects of IL-6R vs. glucocorticoid receptors during rat hind paw inflammatory pain. NEUROSCIENCE LETTERS 2020 Sep 6;738:135356. (PubMed: 32898615) [IF=2.5]

Application: WB    Species: rat    Sample: spinal cord

Fig. 4. |IL-6-induced signaling pathway test was assessed in the spinal cord by Western blotting, and the IL-6 and sIL-6R concentration levels in the serum and CSF were tested by ELISA. A–H: Levels of IL-6, IL-6Rα, gp130, JAK2, pJAK2, STAT3, and pSTAT3 proteins in the spinal cord following three consecutive days of i.th. injection of anti-IL-6R (20 μg/d), sgp130 (20 μg/d), and Dexamethasone (20 μg/d).

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