Product: STAT6 Antibody
Catalog: AF6302
Description: Rabbit polyclonal antibody to STAT6
Application: WB IHC IF/ICC IP
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 94kDa; 94kD(Calculated).
Uniprot: P42226
RRID: AB_2835151

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
STAT6 Antibody detects endogenous levels of total STAT6.
RRID:
AB_2835151
Cite Format: Affinity Biosciences Cat# AF6302, RRID:AB_2835151.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

12S1644; D12S1644; IL 4 STAT; IL-4 Stat; IL4 STAT; Interleukin 4 Induced; Interleukin 4 Induced Transcription Factor IL4 STAT; Signal transducer and activator of transcription 6; Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced; Signal Transducer And Activator Of Transcription 6 Nirs Variant 1; Signal transducer and activator of transcription 6, interleukin 4 induced; STAT 6; STAT interleukin4 induced; STAT, interleukin4 induced; Stat6; STAT6_HUMAN; STAT6B; STAT6C; Transcription factor IL 4 STAT;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
STAT6 transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4.
Sequence:
MSLWGLVSKMPPEKVQRLYVDFPQHLRHLLGDWLESQPWEFLVGSDAFCCNLASALLSDTVQHLQASVGEQGEGSTILQHISTLESIYQRDPLKLVATFRQILQGEKKAVMEQFRHLPMPFHWKQEELKFKTGLRRLQHRVGEIHLLREALQKGAEAGQVSLHSLIETPANGTGPSEALAMLLQETTGELEAAKALVLKRIQIWKRQQQLAGNGAPFEESLAPLQERCESLVDIYSQLQQEVGAAGGELEPKTRASLTGRLDEVLRTLVTSCFLVEKQPPQVLKTQTKFQAGVRFLLGLRFLGAPAKPPLVRADMVTEKQARELSVPQGPGAGAESTGEIINNTVPLENSIPGNCCSALFKNLLLKKIKRCERKGTESVTEEKCAVLFSASFTLGPGKLPIQLQALSLPLVVIVHGNQDNNAKATILWDNAFSEMDRVPFVVAERVPWEKMCETLNLKFMAEVGTNRGLLPEHFLFLAQKIFNDNSLSMEAFQHRSVSWSQFNKEILLGRGFTFWQWFDGVLDLTKRCLRSYWSDRLIIGFISKQYVTSLLLNEPDGTFLLRFSDSEIGGITIAHVIRGQDGSPQIENIQPFSAKDLSIRSLGDRIRDLAQLKNLYPKKPKDEAFRSHYKPEQMGKDGRGYVPATIKMTVERDQPLPTPELQMPTMVPSYDLGMAPDSSMSMQLGPDMVPQVYPPHSHSIPPYQGLSPEESVNVLSAFQEPHLQMPPSLGQMSLPFDQPHPQGLLPCQPQEHAVSSPDPLLCSDVTMVEDSCLSQPVTAFPQGTWIGEDIFPPLLPPTEQDLTKLLLEGQGESGGGSLGAQPLLQPSHYGQSGISMSHMDLRANPSW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42226 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S8 Phosphorylation
K9 Ubiquitination
K107 Ubiquitination
K108 Ubiquitination
K124 Ubiquitination
K129 Ubiquitination
K252 Ubiquitination
K277 Ubiquitination
K284 Ubiquitination
K288 Ubiquitination
K307 Ubiquitination
K319 Ubiquitination
K361 Ubiquitination
S407 Phosphorylation Q9UHD2 (TBK1)
K450 Ubiquitination
K504 Ubiquitination
T525 Phosphorylation
K595 Ubiquitination
K613 Ubiquitination
K621 Ubiquitination
K630 Ubiquitination
K636 Ubiquitination
Y641 Phosphorylation Q9UHD2 (TBK1)
T645 Phosphorylation
K647 Ubiquitination
S707 Phosphorylation P45983 (MAPK8)
S756 Phosphorylation
S817 Phosphorylation

Research Backgrounds

Function:

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

PTMs:

Tyrosine phosphorylated on Tyr-641 following stimulation by IL4/interleukin-4. Tyrosine phosphorylated following stimulation by IL3/interleukin-3 (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling.

Mono-ADP-ribosylated by PARP14.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a homodimer or a heterodimer with a related family member (By similarity). Interacts with NCOA1 via its C-terminal LXXLL motif.

Family&Domains:

Belongs to the transcription factor STAT family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

References

1). Wang D et al. CTLA4Ig/VISTAIg combination therapy selectively induces CD4+ T cell-mediated immune tolerance by targeting the SOCS1 signaling pathway in porcine islet xenotransplantation. IMMUNOLOGY 2022 Mar 9. (PubMed: 35263451) [IF=6.4]

2). Ai-Hua Xu et al. Poly(ADP-ribose) polymerase family member 14 promotes functional recovery after spinal cord injury through regulating microglia M1/M2 polarization via STAT1/6 pathway. Neural Regeneration Research (PubMed: 36751810) [IF=6.1]

Application: WB    Species: Mouse    Sample:

Figure 5 PARP14 deficiency activates the STAT1 pathway but blocks the STAT6 pathway in mice 7 days post-SCI. (A) Representative images and quantitative analysis showing p-STAT1 (Try701)+/Iba1+ immunofluorescence staining. Lv-shPARP14 injection further promoted SCI-induced STAT1 pathway activation. White arrows indicate p-STAT1 (Try701)+ (green, FITC-labeled)/Iba1+ (red, Cy3-labeled, microglia marker) cells. (B) Representative images and quantitative analysis showing p-STAT6 (Tyr641)+/Iba1+ immunofluorescence staining. Lv-shPARP14 injection inhibited SCI-induced STAT6 pathway activation. White arrows indicated p-STAT6 (Tyr641)+ (green, FITC-labeled)/Iba1+ (red, Cy3-labeled) cells. Scale bars: 50 µm. (C) Relative protein levels of p-STAT1 (Try701), and p-STAT6 (Tyr641) in each group were detected by western blot analysis. p-STAT1 (Try701) expression was increased by Lv-shPARP14 injection, but p-STAT6 (Tyr641) expression was decreased by PARP14 silencing. Values are shown as mean ± SD (n = 6). **P < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test). Images were taken from the gray matter ventral horn at the injury site. Spinal cord tissues from the injury site were used for western blot detection. DAPI: 4′,6-Diamidino-2-phenylindole; Iba1: ionized calcium-binding adaptor molecule 1; PARP14: poly(ADP-ribose)polymerase, member 14; SCI: spinal cord injury; STAT1: signal transducer and activator of transcription.

3). Ding S et al. Dedicator of Cytokinesis 2 (DOCK2) Silencing Protects Against Cerebral Ischemia/Reperfusion by Modulating Microglia Polarization via the Activation of the STAT6 Signaling Pathway. NEUROSCIENCE 2022 Apr 5;S0306-4522(22)00172-5. (PubMed: 35395356) [IF=3.3]

4). Li L et al. TRPM7 modulates macrophage polarization by STAT1/STAT6 pathways in RAW264. 7 cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2020 Nov 3;S0006-291X(20)31985-9. (PubMed: 33153718) [IF=3.1]

Application: WB    Species: Mouse    Sample: raw264.7 cells

Fig. 4. TRPM7 can mediate the macrophage polarization through transcription factors such as STAT1 and STAT6. (A and C) The protein levels of p-STAT1, STAT1, p-STAT6 and STAT6 were detected by Western blot assay. N ¼ 3; mean ± SD. (B and D) The ratios of p-STAT1 to STAT1 and the ratios of p-STAT6 to STAT6. N ¼ 3; mean ± SD. Control, untreated RAW264.7 cells. 2-APB, raw264.7 cells with 2-APB for 24 h. M1, RAW264.7 cells was stimulated with LPS- IFN-g for 24 h. M1þ2-APB, RAW264.7 cells was pretreated with 2-APB for 2 h and stimulated with LPS-IFN-g for 24 h. M2, RAW264.7 cells was stimulated with IL-4 for 24 h. M2þ2-APB, RAW264.7 cells was pretreated with 2-APB for 2 h and with IL-4 for 24 h. NC, RAW264.7 cells transfected with scrambled sequence. siRNA3, RAW264.7 cells transfected with siRNA3. M1þsiRNA3, RAW264.7 cells transfected with siRNA3-TRPM7 and stimulated with LPS-IFN-g for 24 h. M2þsiRNA3, RAW264.7 cells transfected with TRPM7-siRNA3 and stimulated with IL-4 for 24 h**P < 0.01, ***P < 0.0001 versus control; *P < 0.05, **P < 0.01, versus M1; &P < 0.05 versus M2.

5). Xu L et al. Scorpion venom polypeptide governs alveolar macrophage M1/M2 polarization to alleviate pulmonary fibrosis. Tissue and Cell 2022 Sep 17;79:101939 (PubMed: 36179453) [IF=2.6]

6). Yuan C et al. Modulation of Wnt/β-catenin signaling in IL-17A-mediated macrophage polarization of RAW264. 7 cells. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH 2020 Jun 19;53(8):e9488. (PubMed: 32578719) [IF=2.3]

Application: WB    Species: mouse    Sample: RAW264.7 cells

Figure 6. |Stat signaling is involved in Wnt3A and IL-17A-mediated macrophage polarization in RAW264.7 cells. A, Representative images of immunoblots of indicated proteins of Stat signaling cascade in cells treated with different conditions. Semi-quantitative analysis of the expression of proteins in (A) showing fold changes of protein abundance of p-STAT1 (B), p-STAT3 (C), BCL-XL (D), p21(E), SOCS3 (F), p-STAT6 (G), and STAT6 (H) in cells treated with Wnt3a-CM and/or XAV939 in the presence or absence of rhIL-17A determined by densitometry assay using ImageJ software Fiji.

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