Product: RACGAP1 Antibody
Catalog: AF6482
Description: Rabbit polyclonal antibody to RACGAP1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 72kDa; 71kD(Calculated).
Uniprot: Q9H0H5
RRID: AB_2835162

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%), Chicken(100%), Xenopus(86%)
RACGAP1 Antibody detects endogenous levels of total RACGAP1.
Cite Format: Affinity Biosciences Cat# AF6482, RRID:AB_2835162.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


CYK4; GAP; Gap1; GTPase activating protein; HsCYK-4; ID GAP; KIAA1478; Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolg; Protein CYK4 homolog; Rac GTPase activating protein 1; Rac GTPase-activating protein 1; RACGAP 1; Racgap1; RGAP1_HUMAN;



Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H0H5 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K53 Ubiquitination
K60 Sumoylation
K60 Ubiquitination
K71 Ubiquitination
K84 Ubiquitination
K95 Ubiquitination
S112 Phosphorylation
S123 Phosphorylation
K141 Ubiquitination
S144 Phosphorylation Q96GD4 (AURKB)
T145 Phosphorylation Q96GD4 (AURKB)
S149 Phosphorylation P53350 (PLK1)
S151 Phosphorylation
S154 Phosphorylation
S157 Phosphorylation P53350 (PLK1)
T161 Phosphorylation
S164 Phosphorylation P53350 (PLK1)
S169 Phosphorylation
S170 Phosphorylation P53350 (PLK1)
K173 Ubiquitination
S185 Phosphorylation Q96GD4 (AURKB)
T186 Phosphorylation Q96GD4 (AURKB)
S187 Phosphorylation Q96GD4 (AURKB)
K199 Sumoylation
K199 Ubiquitination
T201 Phosphorylation
S203 Phosphorylation O14757 (CHEK1)
S206 Phosphorylation
S214 Phosphorylation P53350 (PLK1)
S234 Phosphorylation
Y241 Phosphorylation
T243 Phosphorylation
K248 Sumoylation
K248 Ubiquitination
T249 Phosphorylation Q96GD4 (AURKB) , P31751 (AKT2)
T251 Phosphorylation
S257 Phosphorylation
S259 Phosphorylation
T260 Phosphorylation P53350 (PLK1)
S274 Phosphorylation
T277 Phosphorylation
S280 Phosphorylation
K292 Ubiquitination
K296 Sumoylation
K296 Ubiquitination
S299 Phosphorylation
K305 Ubiquitination
K311 Sumoylation
S313 Phosphorylation
T342 Phosphorylation
S359 Phosphorylation
S387 Phosphorylation Q96GD4 (AURKB)
K404 Ubiquitination
T405 Phosphorylation
S410 Phosphorylation Q96GD4 (AURKB)
K411 Ubiquitination
S420 Phosphorylation
K423 Ubiquitination
K430 Ubiquitination
K490 Ubiquitination
K522 Ubiquitination
S533 Phosphorylation
S540 Phosphorylation
S563 Phosphorylation
T564 Phosphorylation
T567 Phosphorylation
K571 Sumoylation
S573 Phosphorylation
T579 Phosphorylation
T580 Phosphorylation
K587 Methylation
K587 Ubiquitination
T588 Phosphorylation P06493 (CDK1)
S590 Phosphorylation
S591 Phosphorylation
S592 Phosphorylation
S593 Phosphorylation
S595 Phosphorylation
S600 Phosphorylation
T601 Phosphorylation
T603 Phosphorylation
K604 Ubiquitination
T606 Phosphorylation
S611 Phosphorylation
K614 Methylation
K614 Ubiquitination
S615 Phosphorylation
S628 Phosphorylation
K632 Acetylation

Research Backgrounds


Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.


Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle. Cytoplasmic vesicle>Secretory vesicle>Acrosome. Cleavage furrow. Midbody>Midbody ring. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody ring, also called Flemming body. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.

Subunit Structure:

Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Associates with alpha-, beta- and gamma-tubulin and microtubules. Interacts via its Rho-GAP domain with RND2. Associates with AURKB during M phase. Interacts via its Rho-GAP domain and basic region with PRC1. The interaction with PRC1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts with SLC26A8 via its N-terminus. Interacts with RAB11FIP3. Interacts with ECT2; the interaction is direct, occurs at anaphase and during cytokinesis in a microtubule-dependent manner and is enhanced by phosphorylation by PLK1. Interacts with KIF23; the interaction is direct.


The coiled coil region is indispensible for localization to the midbody during cytokinesis.

The phorbol-ester/DAG-type zinc finger domain mediates interaction with membranes enriched in phosphatidylinositol 3,4,5-trisphosphate and is required during mitotic cytokinesis for normal attachment of the midbody to the cell membrane.

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