EMP3 Antibody - #DF14661

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Product: EMP3 Antibody
Catalog: DF14661
Description: Rabbit polyclonal antibody to EMP3
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 18kD(Calculated).
Uniprot: P54852

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
EMP3 Antibody detects endogenous levels of EMP3.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EMP 3; EMP-3; Emp3; EMP3_HUMAN; Epithelial membrane protein 3; Hematopoietic neural membrane protein 1; Hematopoietic neural membrane protein; HGNC:3335; HNMP 1; HNMP-1; Protein YMP; YMP; YMP protein;

Immunogens

Immunogen:

A synthesized peptide derived from human EMP3.

Uniprot:

P54852(EMP3_HUMAN) >>Visit HPA database.

Gene(ID):
EMP3(2014)
Sequence:
MSLLLLVVSALHILILILLFVATLDKSWWTLPGKESLNLWYDCTWNNDTKTWACSNVSENGWLKAVQVLMVLSLILCCLSFILFMFQLYTMRRGGLFYATGLCQLCTSVAVFTGALIYAIHAEEILEKHPRGGSFGYCFALAWVAFPLALVSGIIYIHLRKRE

Research Backgrounds

Function:

Probably involved in cell proliferation and cell-cell interactions.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the PMP-22/EMP/MP20 family.

References

1). EMP3 as a prognostic biomarker correlates with EMT in GBM. BMC cancer, 2024 (PubMed: 38229014) [IF=3.8]

Application: WB    Species: human    Sample:

Fig. 5 Knockdown of EMP3 inhibits the malignant phenotypes of glioblastomas (A) and (B). The expression of EMP3 was significantly reduced after transfection of U87 and U251 cells for 72 h cultured with siRNA, and GAPDH was used as an internal control (C) and (D). The proliferation rate of U87 and U251 cells in the si-EMP3 group was significantly lower than that of the NC group at 24 h, 48 h, 72 h (E). The number of migrating U87 and U251 cells in the si-EMP3 group was lower than that in the NC group at 72-96 h (F). Wound healing results indicated the migration capacity of the si-NRP1 group was significantly lower than that of the NC group at 72-96 h.The knockdown efficiency of EMP3 in U251 glioma cells are detected by western blot (G). Cells were divided into four groups, NC (negtive control) group, siRNA EMP3-1, siRNA EMP3-2 and siRNA EMP3-3 group. U251 cells were lysis after siRNA incubation for 72 h. All of the full-length blots/gels of WB are presented in Supplementary Figure 1A and B. *p 

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