NENF Antibody - #DF14667

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Product: NENF Antibody
Catalog: DF14667
Description: Rabbit polyclonal antibody to NENF
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 19kD(Calculated).
Uniprot: Q9UMX5

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
NENF Antibody detects endogenous levels of NENF.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cell growth inhibiting protein 47; Cell immortalization-related protein 2; CIR2; Nenf; NENF_HUMAN; Neudesin; Neuron derived neurotrophic factor; Neuron-derived neurotrophic factor; SCIRP10; SCIRP10 related protein; Secreted protein of unknown function; Spinal cord injury related protein 10; SPUF; SPUF protein;

Immunogens

Immunogen:

A synthesized peptide derived from human NENF.

Uniprot:

Q9UMX5(NENF_HUMAN) >>Visit HPA database.

Gene(ID):
NENF(29937)
Expression:
Q9UMX5 NENF_HUMAN:

Ubiquitously expressed with high expression in heart. Over-expressed in various tumors including carcinomas of the uterine cervix, lymphoma, colon, lung, skin and leukemia, as well as carcinoma of the breast.

Sequence:
MVGPAPRRRLRPLAALALVLALAPGLPTARAGQTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF

Research Backgrounds

Function:

Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural progenitor cells at the embryonic stage and inhibits differentiation of astrocytes (By similarity). Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways (By similarity). Neurotrophic activity is enhanced by binding to heme (By similarity). Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity).

Subcellular Location:

Secreted>Extracellular space. Mitochondrion. Endoplasmic reticulum.
Note: Localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with high expression in heart. Over-expressed in various tumors including carcinomas of the uterine cervix, lymphoma, colon, lung, skin and leukemia, as well as carcinoma of the breast.

Subunit Structure:

Interacts with PINK1 and PARK7.

Family&Domains:

The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.

Belongs to the cytochrome b5 family. MAPR subfamily.

Restrictive clause

 

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