PROM2 Antibody | Affinity Biosciences

IHC
Cites

1/3

PROM2 Antibody - DF14670 at 1/100 staining mouse testis tissue by IHC-P.

Product:	PROM2 Antibody
Catalog:	DF14670
Description:	Rabbit polyclonal antibody to PROM2
Application:	IHC
Cited expt.:	IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	92kD(Calculated).
Uniprot:	Q8N271

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

PROM2 Antibody detects endogenous levels of PROM2.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

hPROML2; MGC138714; OTTHUMP00000160939; OTTHUMP00000207357; PROM 2; PROM-2; PROM2; PROM2_HUMAN; Prominin like protein 2; Prominin related protein; Prominin-2; Prominin-like protein 2; PROML2;

Immunogens

Immunogen:

A synthesized peptide derived from human PROM2.

Uniprot:

Q8N271(PROM2_HUMAN) >>Visit HPA database.

Gene(ID):

PROM2(150696)

Expression:

Q8N271 PROM2_HUMAN:

Present in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.

Sequence:

Q8N271 PROM2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKHTLALLAPLLGLGLGLALSQLAAGATDCKFLGPAEHLTFTPAARARWLAPRVRAPGLLDSLYGTVRRFLSVVQLNPFPSELVKALLNELASVKVNEVVRYEAGYVVCAVIAGLYLLLVPTAGLCFCCCRCHRRCGGRVKTEHKALACERAALMVFLLLTTLLLLIGVVCAFVTNQRTHEQMGPSIEAMPETLLSLWGLVSDVPQELQAVAQQFSLPQEQVSEELDGVGVSIGSAIHTQLRSSVYPLLAAVGSLGQVLQVSVHHLQTLNATVVELQAGQQDLEPAIREHRDRLLELLQEARCQGDCAGALSWARTLELGADFSQVPSVDHVLHQLKGVPEANFSSMVQEENSTFNALPALAAMQTSSVVQELKKAVAQQPEGVRTLAEGFPGLEAASRWAQALQEVEESSRPYLQEVQRYETYRWIVGCVLCSVVLFVVLCNLLGLNLGIWGLSARDDPSHPEAKGEAGARFLMAGVGLSFLFAAPLILLVFATFLVGGNVQTLVCQSWENGELFEFADTPGNLPPSMNLSQLLGLRKNISIHQAYQQCKEGAALWTVLQLNDSYDLEEHLDINQYTNKLRQELQSLKVDTQSLDLLSSAARRDLEALQSSGLQRIHYPDFLVQIQRPVVKTSMEQLAQELQGLAQAQDNSVLGQRLQEEAQGLRNLHQEKVVPQQSLVAKLNLSVRALESSAPNLQLETSDVLANVTYLKGELPAWAARILRNVSECFLAREMGYFSQYVAWVREEVTQRIATCQPLSGALDNSRVILCDMMADPWNAFWFCLAWCTFFLIPSIIFAVKTSKYFRPIRKRLSSTSSEETQLFHIPRVTSLKL

Research Backgrounds

Q8N271_PROM2_HUMAN

PTMs:

Glycosylated.

Subcellular Location:

Apical cell membrane>Multi-pass membrane protein. Basolateral cell membrane>Multi-pass membrane protein. Cell projection>Microvillus membrane>Multi-pass membrane protein. Cell projection>Cilium membrane>Multi-pass membrane protein.
Note: Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells (By similarity).

Tissue Specificity:

Family&Domains:

Belongs to the prominin family.

References

1). Inhibition of HOXC11 by artesunate induces ferroptosis and suppresses ovarian cancer progression through transcriptional regulation of the PROM2/PI3K/AKT pathway. World journal of surgical oncology, 2024 (PubMed: 39380001) [IF=3.2]

Application: WB Species: Mouse Sample:

Fig. 1 Identification of ferroptosis-related genes induced by artesunate (ART) by RNA-seq (A) Volcano plot showed differentially expressed genes (DEGs) between control and ART-treated ES2 cells (|log2FC| > 1 and p value

Application: IHC Species: Mouse Sample:

Fig. 7 Artesunate (ART) induced ferroptosis-mediated tumor inhibition by suppressing the HOXC11/PROM2/PI3K/AKT pathway in a SKOV3 xenograft mouse model (A) Scheme of the experimental design and timeline for the tumor model. SKOV3 cells (5 × 106 cells for each mouse) were inoculated into BALB/c-nude mice subcutaneously. Tumor-bearing mice were treated with vehicle (5% DMSO) or ART at 70 mg/kg daily for 25 days (n = 6 for each group). Body weights and tumor volumes were monitored every three days. B Images of xenograft nude mice and tumor mass. C Curve of tumor volume. D Curve of body weight. E Tumor weight. The superoxide dismutase (SOD) activity (F), glutathione (GSH) levels (G) and malondialdehyde (MDA) levels (H) were measured in the tumor tissue of nude mice. I IHC staining was performed to detect the expression of HOXC11, PROM2 and GPX4 in the tumor tissues of xenografted mice. J Western blot analysis of HOXC11, PROM2, GPX4, p-PI3K, PI3KCG, p-AKT and AKT in tumor tissue samples obtained from xenograft mice treated with vehicle or ART. GAPDH was used as a loading control. Data were analyzed as the mean ± SD of three independent experiments. *p

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PROM2 Antibody - #DF14670