Product: TOP2B Antibody
Catalog: AF0283
Description: Rabbit polyclonal antibody to TOP2B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Sheep, Dog, Chicken
Mol.Wt.: 183kDa; 183kD(Calculated).
Uniprot: Q02880
RRID: AB_2833451

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(93%), Sheep(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
TOP2B Antibody detects endogenous levels of total TOP2B.
RRID:
AB_2833451
Cite Format: Affinity Biosciences Cat# AF0283, RRID:AB_2833451.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antigen MLAA 44; beta isozyme; DNA topoisomerase 2 beta; DNA topoisomerase 2-beta; DNA topoisomerase II 180 kD; DNA topoisomerase II; DNA topoisomerase II beta; DNA topoisomerase II beta isozyme; Top 2; TOP 2B; TOP IIB; Top2 beta; TOP2B; TOP2B_HUMAN; Top2beta; TOPIIB; Topo II beta; Topoisomerase (DNA) II beta 180kDa; Topoisomerase (DNA) II beta; Topoisomerase IIb; U937 associated antigen;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
TOP2B Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the type II topoisomerase family.
Sequence:
MAKSGGCGAGAGVGGGNGALTWVTLFDQNNAAKKEESETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGRARKEVKYFAESDEEEDDVDFAMFN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Dog
100
Bovine
93
Chicken
80
Xenopus
67
Pig
0
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02880 As Substrate

Site PTM Type Enzyme
Sumoylation
S4 Phosphorylation
K34 Sumoylation
K34 Ubiquitination
S37 Phosphorylation
K42 Ubiquitination
S45 Phosphorylation
S46 Phosphorylation
Y103 Phosphorylation
K117 Ubiquitination
K121 Ubiquitination
K144 Ubiquitination
K152 Sumoylation
K152 Ubiquitination
K155 Ubiquitination
Y157 Phosphorylation
Y172 Phosphorylation
K178 Ubiquitination
K189 Ubiquitination
K205 Ubiquitination
K212 Ubiquitination
K221 Ubiquitination
K228 Sumoylation
K228 Ubiquitination
Y235 Phosphorylation
K246 Ubiquitination
K254 Ubiquitination
Y265 Phosphorylation
K275 Acetylation
K282 Ubiquitination
Y291 Phosphorylation
K297 Ubiquitination
K299 Ubiquitination
K308 Ubiquitination
K342 Ubiquitination
Y349 Phosphorylation
K365 Acetylation
K367 Acetylation
K367 Ubiquitination
K373 Acetylation
K373 Sumoylation
K373 Ubiquitination
K407 Ubiquitination
K412 Methylation
K412 Ubiquitination
K418 Ubiquitination
K421 Methylation
K439 Methylation
K439 Ubiquitination
K452 Acetylation
Y453 Phosphorylation
K461 Sumoylation
K461 Ubiquitination
K471 Ubiquitination
K487 Ubiquitination
S488 Phosphorylation
S492 Phosphorylation
K510 Sumoylation
K510 Ubiquitination
K520 Ubiquitination
K533 Ubiquitination
Y539 Phosphorylation
K540 Ubiquitination
K541 Ubiquitination
K550 Ubiquitination
K556 Ubiquitination
K587 Methylation
K600 Sumoylation
K600 Ubiquitination
K605 Ubiquitination
K620 Ubiquitination
K621 Acetylation
K627 Ubiquitination
K635 Ubiquitination
T639 Phosphorylation
S640 Phosphorylation
K643 Sumoylation
K643 Ubiquitination
K646 Sumoylation
K646 Ubiquitination
K676 Ubiquitination
K683 Sumoylation
K683 Ubiquitination
R693 Methylation
Y707 Phosphorylation
K712 Ubiquitination
K722 Ubiquitination
S730 Phosphorylation
S735 Phosphorylation
S738 Phosphorylation
K744 Ubiquitination
K749 Ubiquitination
K756 Ubiquitination
K848 Ubiquitination
Y851 Phosphorylation
K884 Ubiquitination
Y888 Phosphorylation
Y913 Phosphorylation
Y928 Phosphorylation
K970 Ubiquitination
K979 Acetylation
K979 Ubiquitination
K988 Ubiquitination
K992 Acetylation
K992 Ubiquitination
K1008 Acetylation
K1008 Ubiquitination
K1032 Sumoylation
K1032 Ubiquitination
K1068 Ubiquitination
K1079 Ubiquitination
K1083 Ubiquitination
S1090 Phosphorylation
K1092 Ubiquitination
K1109 Ubiquitination
S1131 Phosphorylation
S1132 Phosphorylation
S1133 Phosphorylation
S1135 Phosphorylation
T1137 Phosphorylation
S1139 Phosphorylation
K1164 Ubiquitination
S1212 Phosphorylation
K1214 Ubiquitination
K1227 Sumoylation
K1227 Ubiquitination
T1233 Phosphorylation
S1236 Phosphorylation
T1247 Phosphorylation
K1250 Sumoylation
K1250 Ubiquitination
K1255 Acetylation
K1259 Acetylation
K1260 Acetylation
K1262 Ubiquitination
T1267 Phosphorylation
K1271 Sumoylation
S1279 Phosphorylation
T1292 Phosphorylation
K1299 Ubiquitination
K1302 Ubiquitination
K1307 Acetylation
T1317 Phosphorylation
K1323 Acetylation
K1323 Ubiquitination
K1327 Acetylation
K1328 Acetylation
S1336 Phosphorylation
S1340 Phosphorylation
S1342 Phosphorylation
S1344 Phosphorylation
T1349 Phosphorylation
S1358 Phosphorylation
R1362 Methylation
R1367 Methylation
Y1370 Phosphorylation
T1371 Phosphorylation
S1375 Phosphorylation
S1400 Phosphorylation
T1403 Phosphorylation
S1413 Phosphorylation
Y1421 Phosphorylation
T1422 Phosphorylation
S1424 Phosphorylation P06493 (CDK1)
S1428 Phosphorylation
T1431 Phosphorylation
K1434 Acetylation
S1435 Phosphorylation
K1440 Sumoylation
K1440 Ubiquitination
S1441 Phosphorylation
S1449 Phosphorylation
S1452 Phosphorylation
Y1453 Phosphorylation
S1454 Phosphorylation
K1456 Sumoylation
K1456 Ubiquitination
S1457 Phosphorylation
S1461 Phosphorylation
S1466 Phosphorylation
S1471 Phosphorylation
S1473 Phosphorylation
S1476 Phosphorylation
K1482 Ubiquitination
K1486 Acetylation
S1501 Phosphorylation
S1522 Phosphorylation
S1524 Phosphorylation
S1526 Phosphorylation
S1550 Phosphorylation
S1552 Phosphorylation
K1574 Ubiquitination
T1575 Phosphorylation
S1576 Phosphorylation
S1581 Phosphorylation
S1588 Phosphorylation
T1592 Phosphorylation
S1596 Phosphorylation
T1600 Phosphorylation
Y1609 Phosphorylation
S1613 Phosphorylation

Research Backgrounds

Function:

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

Subcellular Location:

Cytoplasm. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with KIAA1210 (By similarity).

Family&Domains:

Belongs to the type II topoisomerase family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

References

1). Wang X et al. Effect of verapamil in the reversal of doxorubicin chemotherapy resistance in advanced gastric cancer. European Review for Medical and Pharmacological Sciences 2020 Jul;24(14):7753-7763. (PubMed: 32744702) [IF=3.3]

Application: WB    Species: human    Sample: GC cells

Figure 2. |Evaluation of candidate gene/protein expression in GC cells. A, RT-qPCR detection of candidate gene expression in GC cells. Independent experiments were performed during the whole in vitro study. B, Western blot analysis of candidate protein expression levels in GC cells.

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