NELFE Antibody | Affinity Biosciences

IF/ICC

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NELFE Antibody - DF14763 staining Hela cells by IF/ICC.

Product:	NELFE Antibody
Catalog:	DF14763
Description:	Rabbit polyclonal antibody to NELFE
Application:	IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	43kD(Calculated).
Uniprot:	P18615

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

NELFE Antibody detects endogenous levels of NELFE.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Major histocompatibility complex gene RD; Negative elongation factor E; Negative elongation factor polypeptide E; NELF e; NELF-E; Nelfe; NELFE_HUMAN; Nuclear protein; RD; RD protein; RD RNA binding protein; RDP; RNA-binding protein RD;

Immunogens

Immunogen:

A synthesized peptide derived from human NELFE.

Uniprot:

P18615(NELFE_HUMAN) >>Visit HPA database.

Gene(ID):

NELFE(7936)

Expression:

P18615 NELFE_HUMAN:

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Sequence:

P18615 NELFE_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Research Backgrounds

P18615_NELFE_HUMAN

Function:

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA.

(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.

PTMs:

Sumoylated.

Subcellular Location:

Nucleus.

Tissue Specificity:

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Subunit Structure:

The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE. Interacts with NELFB (By similarity).

(Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.

Family&Domains:

The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.

Belongs to the RRM NELF-E family.

Restrictive clause

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For Research Use Only.
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NELFE Antibody - #DF14763