SCYL3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

SCYL3 Antibody - Western blot analysis of extracts from various samples, using SCYL3 Antibody.

SCYL3 Antibody - DF14841 at 1/100 staining rat muscle tissue by IHC-P.

SCYL3 Antibody - DF14841 staining Hela cells by IF/ICC.

Product:	SCYL3 Antibody
Catalog:	DF14841
Description:	Rabbit polyclonal antibody to SCYL3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	83kD(Calculated).
Uniprot:	Q8IZE3

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

SCYL3 Antibody detects endogenous levels of SCYL3.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Ezrin binding protein PACE 1; Ezrin-binding protein PACE-1; PACE 1; PACE1; PACE1_HUMAN; Protein associating with the carboxyl terminal domain of ezrin; Protein-associating with the carboxyl-terminal domain of ezrin; SCY1 like 3 (S. cerevisiae); SCY1 like 3; SCY1 like protein 3; SCY1 like pseudokinase 3; SCY1-like protein 3; SCYL 3; SCYL3;

Immunogens

Immunogen:

A synthesized peptide derived from human SCYL3.

Uniprot:

Q8IZE3(PACE1_HUMAN) >>Visit HPA database.

Gene(ID):

SCYL3(57147)

Expression:

Q8IZE3 PACE1_HUMAN:

Ubiquitously expressed.

Sequence:

Q8IZE3 PACE1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSENSALKSYTLREPPFTLPSGLAVYPAVLQDGKFASVFVYKRENEDKVNKAAKHLKTLRHPCLLRFLSCTVEADGIHLVTERVQPLEVALETLSSAEVCAGIYDILLALIFLHDRGHLTHNNVCLSSVFVSEDGHWKLGGMETVCKVSQATPEFLRSIQSIRDPASIPPEEMSPEFTTLPECHGHARDAFSFGTLVESLLTILNEQVSADVLSSFQQTLHSTLLNPIPKCRPALCTLLSHDFFRNDFLEVVNFLKSLTLKSEEEKTEFFKFLLDRVSCLSEELIASRLVPLLLNQLVFAEPVAVKSFLPYLLGPKKDHAQGETPCLLSPALFQSRVIPVLLQLFEVHEEHVRMVLLSHIEAYVEHFTQEQLKKVILPQVLLGLRDTSDSIVAITLHSLAVLVSLLGPEVVVGGERTKIFKRTAPSFTKNTDLSLEDSPMCVVCSHHSQISPILENPFSSIFPKCFFSGSTPINSKKHIQRDYYNTLLQTGDPFSQPIKFPINGLSDVKNTSEDSENFPSSSKKSEEWPDWSEPEEPENQTVNIQIWPREPCDDVKSQCTTLDVEESSWDDCEPSSLDTKVNPGGGITATKPVTSGEQKPIPALLSLTEESMPWKSSLPQKISLVQRGDDADQIEPPKVSSQERPLKVPSELGLGEEFTIQVKKKPVKDPEMDWFADMIPEIKPSAAFLILPELRTEMVPKKDDVSPVMQFSSKFAAAEITEGEAEGWEEEGELNWEDNNW

Research Backgrounds

Q8IZE3_PACE1_HUMAN

Function:

May play a role in regulating cell adhesion/migration complexes in migrating cells.

PTMs:

May be myristoylated; myristoylation may target it to Golgi compartment.

Phosphorylated.

Subcellular Location:

Cytoplasm. Golgi apparatus. Cell projection>Lamellipodium.
Note: Colocalized with EZR/VIL2, actin and CD44 in lamellipodia.

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with EZR/VIL2 C-terminal domain.

Family&Domains:

The protein kinase domain is predicted to be catalytically inactive.

Belongs to the protein kinase superfamily.

Restrictive clause

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SCYL3 Antibody - #DF14841