Product: DPH1 Antibody
Catalog: DF14907
Description: Rabbit polyclonal antibody to DPH1
Application: WB IHC
Reactivity: Human
Mol.Wt.: 49kD(Calculated).
Uniprot: Q9BZG8

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
DPH1 Antibody detects endogenous levels of DPH1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Candidate tumor suppressor in ovarian cancer 1; Diphthamide biosynthesis protein 1; Diphthamide biosynthesis protein 2 homolog like 1; Diphthamide biosynthesis protein 2 homolog-like 1; Diphthamide biosynthesis protein 2 like; Diphthamide biosynthesis protein 2-like; Diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces) like 1; Diptheria toxin resistance protein required for diphthamide biosynthesis like 1; DPH like 1 (S. cerevisiae); DPH like 1; DPH-like 1; DPH1; DPH1 homolog (S. cerevisiae); DPH1 homolog; DPH1_HUMAN; DPH2 like 1; DPH2-like 1; DPH2L; DPH2L1; FLJ33211; HsDph1; Ovarian cancer associated gene 1; Ovarian cancer associated gene 1 protein; Ovarian cancer-associated gene 1 protein; Ovarian tumor suppressor candidate 1;

Immunogens

Immunogen:

A synthesized peptide derived from human DPH1.

Uniprot:
Gene(ID):
Expression:
Q9BZG8 DPH1_HUMAN:

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, mammary gland, colon, small intestine, testis and ovary. Reduced expression in primary breast and ovarian tumors.

Sequence:
MRRQVMAALVVSGAAEQGGRDGPGRGRAPRGRVANQIPPEILKNPQLQAAIRVLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGADFLVHYGHSCLIPMDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFPPATALALVSTIQFVSTLQAAAQELKAEYRVSVPQCKPLSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQEAIATARSAKSWGLILGTLGRQGSPKILEHLESRLRALGLSFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGSARPPSAVACEDCSCRDEKVAPLAP

Research Backgrounds

Function:

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity). Plays a role in embryonic growth, organogenesis and postnatal survival (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.
Note: Punctate, primarily perinuclear localization.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, mammary gland, colon, small intestine, testis and ovary. Reduced expression in primary breast and ovarian tumors.

Subunit Structure:

Interacts with DPH2 (By similarity). Interacts with RBM8A.

Family&Domains:

Belongs to the DPH1/DPH2 family. DPH1 subfamily.

Restrictive clause

 

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For Research Use Only.
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