NOSIP Antibody - #DF14911

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Product: NOSIP Antibody
Catalog: DF14911
Description: Rabbit polyclonal antibody to NOSIP
Application: WB
Reactivity: Human, Mouse
Mol.Wt.: 35kD; 33kD(Calculated).
Uniprot: Q9Y314

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
NOSIP Antibody detects endogenous levels of NOSIP.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CGI 25; CGI25; CGI25 protein; eNOS interacting protein; Nitric oxide synthase interacting protein;

Immunogens

Immunogen:

A synthesized peptide derived from human NOSIP.

Uniprot:

Q9Y314(NOSIP_HUMAN) >>Visit HPA database.

Gene(ID):
NOSIP(51070)
Expression:
Q9Y314 NOSIP_HUMAN:

Expressed in heart, brain and lung. Present in endothelial cells (at protein level).

Sequence:
MTRHGKNCTAGAVYTYHEKKKDTAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAILEYILHQKKEIARQMKAYEKQRGTRREEQKELQRAASQDHVRGFLEKESAIVSRPLNPFTAKALSGTSPDDVQPGPSVGPPSKDKDKVLPSFWIPSLTPEAKATKLEKPSRTVTCPMSGKPLRMSDLTPVHFTPLDSSVDRVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGDKLTDRDIIVLQRGGTGFAGSGVKLQAEKSRPVMQA

Research Backgrounds

Function:

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle (PubMed:16135813).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain and lung. Present in endothelial cells (at protein level).

Subunit Structure:

Interacts with NOS1 and NOS3. Interacts with PP2A holoenzyme, containing PPP2CA, PPP2CB, PPP2R1A and PPP2R2A subunits (By similarity).

Family&Domains:

The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.

Belongs to the NOSIP family.

Restrictive clause

 

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