PAAF1 Antibody - #DF14934

(2)  
Product: PAAF1 Antibody
Catalog: DF14934
Description: Rabbit polyclonal antibody to PAAF1
Application: IHC IF/ICC
Reactivity: Human
Mol.Wt.: 42kD(Calculated).
Uniprot: Q9BRP4

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
PAAF1 Antibody detects endogenous levels of PAAF1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PAAF; PAAF1; PAAF1_HUMAN; Proteasomal ATPase-associated factor 1; Protein G-16; Rpn14; WD repeat domain 71; WD repeat-containing protein 71; WDR71;

Immunogens

Immunogen:

A synthesized peptide derived from human PAAF1.

Uniprot:

Q9BRP4(PAAF1_HUMAN) >>Visit HPA database.

Gene(ID):
PAAF1(80227)
Expression:
Q9BRP4 PAAF1_HUMAN:

Ubiquitously expressed, with highest levels in kidney, brain and testis.

Sequence:
MAAPLRIQSDWAQALRKDEGEAWLSCHPPGKPSLYGSLTCQGIGLDGIPEVTASEGFTVNEINKKSIHISCPKENASSKFLAPYTTFSRIHTKSITCLDISSRGGLGVSSSTDGTMKIWQASNGELRRVLEGHVFDVNCCRFFPSGLVVLSGGMDAQLKIWSAEDASCVVTFKGHKGGILDTAIVDRGRNVVSASRDGTARLWDCGRSACLGVLADCGSSINGVAVGAADNSINLGSPEQMPSEREVGTEAKMLLLAREDKKLQCLGLQSRQLVFLFIGSDAFNCCTFLSGFLLLAGTQDGNIYQLDVRSPRAPVQVIHRSGAPVLSLLSVRDGFIASQGDGSCFIVQQDLDYVTELTGADCDPVYKVATWEKQIYTCCRDGLVRRYQLSDL

Research Backgrounds

Function:

Inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency. Protects SUPT6H from proteasomal degradation.

Tissue Specificity:

Ubiquitously expressed, with highest levels in kidney, brain and testis.

Subunit Structure:

Interacts with PSMC1, PSMC2, PSMC3, PSMC4, PSMC5 and PSMC6. Interacts with SUPT6H.

(Microbial infection) Interacts with HIV-1 Tat.

Family&Domains:

Belongs to the WD repeat PAAF1/RPN14 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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