Ataxin 1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of Ataxin 1 expression in NIH-3T3 whole cell lysates,The lane on the left was treated with the antigen-specific peptide.

AF6347 staining HepG2 cells by IF/ICC.

Product:	Ataxin 1 Antibody
Catalog:	AF6347
Description:	Rabbit polyclonal antibody to Ataxin 1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	87 kDa; 87kD(Calculated).
Uniprot:	P54253
RRID:	AB_2835203

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(89%), Dog(89%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Ataxin 1 Antibody detects endogenous levels of total Ataxin 1.

RRID:

AB_2835203
Cite Format: Affinity Biosciences Cat# AF6347, RRID:AB_2835203.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

alternative ataxin1; Ataxin-1; ATX1; ATX1_HUMAN; Atxn1; D6S504E; OTTHUMP00000016065; SCA1; Spinocerebellar ataxia type 1 protein;

Immunogens

Immunogen:

Uniprot:

P54253(ATX1_HUMAN) >>Visit HPA database.

Gene(ID):

ATXN1(6310)

Expression:

P54253 ATX1_HUMAN:

Widely expressed throughout the body.

Description:

ataxin-1 is a spinocerebellar ataxia type 1 (SCA1) protein. Expansion of CAG repeats in the SCA1 gene results in an abnormally long polyglutamine tract in the protein, causing polyglutamine-induced neurodegeneration.

Sequence:

P54253 ATX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKSNQERSNECLPPKKREIPATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHGGGRHGPAGTSVELGLQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAYATPQPGTPVSPVQYAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQQQQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVHLHPHQTMIPHTLTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQAKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSDYSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQQAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAPAIVTSSPQFAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAAPPTLPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEDSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKHSKADGLAGSRHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAPFLTKIEPSKPAATRKRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Chicken

100

Horse

Bovine

Sheep

Dog

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54253 As Substrate

P54253

Site	PTM Type	Enzyme	Source
K16	Sumoylation		Uniprot
R53	Methylation		Uniprot
S82	Phosphorylation		Uniprot
S88	Phosphorylation		Uniprot
S91	Phosphorylation		Uniprot
K194	Sumoylation		Uniprot
T235	Phosphorylation		Uniprot
S238	Phosphorylation		Uniprot
Y247	Phosphorylation		Uniprot
S253	Phosphorylation		Uniprot
T257	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
S366	Phosphorylation		Uniprot
S406	Phosphorylation		Uniprot
S408	Phosphorylation		Uniprot
K609	Sumoylation		Uniprot
K696	Sumoylation		Uniprot
S711	Phosphorylation		Uniprot
S739	Phosphorylation		Uniprot
K745	Sumoylation		Uniprot
T769	Phosphorylation		Uniprot
S775	Phosphorylation	P31749 (AKT1) , O75582 (RPS6KA5) , P17612 (PRKACA)	Uniprot
S811	Phosphorylation		Uniprot

Research Backgrounds

P54253_ATX1_HUMAN

Function:

Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. In concert with CIC and ATXN1L, involved in brain development (By similarity).

PTMs:

Ubiquitinated by UBE3A, leading to its degradation by the proteasome. The presence of expanded poly-Gln repeats in spinocerebellar ataxia 1 (SCA1) patients impairs ubiquitination and degradation, leading to accumulation of ATXN1 in neurons and subsequent toxicity.

Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.

Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Colocalizes with USP7 in the nucleus.

Tissue Specificity:

Widely expressed throughout the body.

Subunit Structure:

Homooligomer. Interacts with CIC (By similarity). Interacts with ANP32A, PQBP1, UBQLN4, ATXN1L and USP7. Directly interacts with RBPJ; this interaction is disrupted in the presence of Notch intracellular domain. Competes with ATXN1L for RBPJ-binding. Found in a complex with CIC and ATXN1L (By similarity).

Family&Domains:

The AXH domain is required for interaction with CIC.

Belongs to the ATXN1 family.

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Ataxin 1 Antibody - #AF6347