Product: 14-3-3 zeta Antibody
Catalog: AF6356
Description: Rabbit polyclonal antibody to 14-3-3 zeta
Application: WB IHC IF/ICC IP
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 28kDa; 28kD(Calculated).
Uniprot: P63104
RRID: AB_2835206

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 100ul $280 In stock
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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
14-3-3 zeta Antibody detects endogenous levels of total 14-3-3 zeta.
Cite Format: Affinity Biosciences Cat# AF6356, RRID:AB_2835206.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


14 3 3 delta; 14 3 3 protein zeta/delta; 14 3 3 protein/cytosolic phospholipase A2; 14 3 3 zeta; 14-3-3 protein zeta/delta; 1433Z_HUMAN; Epididymis luminal protein 4; Epididymis secretory protein Li 3; HEL S 3; HEL4; KCIP-1; KCIP1; MGC111427; MGC126532; MGC138156; Phospholipase A2; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide; YWHAD; YWHAZ;


14-3-3 zeta is a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P63104 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K3 Acetylation
K3 Methylation
K3 Ubiquitination
K9 Acetylation
K9 Ubiquitination
K11 Acetylation
K11 Ubiquitination
Y19 Phosphorylation
C25 S-Nitrosylation
K27 Ubiquitination
S28 Phosphorylation
S37 Phosphorylation
S45 Phosphorylation
Y48 Phosphorylation
K49 Acetylation
K49 Methylation
K49 Ubiquitination
S57 Phosphorylation
S58 Phosphorylation P31749 (AKT1) , P49137 (MAPKAPK2) , P17612 (PRKACA) , O00506 (STK25) , Q05655 (PRKCD)
S63 Phosphorylation
S64 Phosphorylation
K68 Acetylation
K68 Ubiquitination
K74 Acetylation
K74 Ubiquitination
K75 Acetylation
K75 Ubiquitination
Y82 Phosphorylation
K85 Acetylation
K85 Methylation
K85 Ubiquitination
T88 Phosphorylation
C94 S-Nitrosylation
S99 Phosphorylation
K103 Ubiquitination
S110 Phosphorylation
S114 Phosphorylation
K115 Acetylation
K115 Ubiquitination
K120 Acetylation
K120 Ubiquitination
K122 Ubiquitination
Y125 Phosphorylation
Y126 Phosphorylation
Y128 Phosphorylation
K138 Acetylation
K138 Ubiquitination
K139 Acetylation
K139 Ubiquitination
S145 Phosphorylation
Y149 Phosphorylation
K157 Acetylation
K157 Ubiquitination
K158 Ubiquitination
Y178 Phosphorylation
S184 Phosphorylation P53779 (MAPK10) , P45984 (MAPK9) , P45983 (MAPK8)
C189 S-Nitrosylation
K193 Ubiquitination
T194 Phosphorylation
T205 Phosphorylation
S207 Phosphorylation
S210 Phosphorylation
Y211 Phosphorylation
K212 Methylation
S214 Phosphorylation
T215 Phosphorylation
T229 Phosphorylation
S230 Phosphorylation
T232 Phosphorylation P48729 (CSNK1A1) , P11274 (BCR)

Research Backgrounds


Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).


The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.

Subcellular Location:

Cytoplasm. Melanosome.
Note: Located to stage I to stage IV melanosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CDK16 and BSPRY (By similarity). Interacts with WEE1 (C-terminal). Interacts with SAMSN1 (By similarity). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm (By similarity). Interacts with Thr-phosphorylated ITGB2 (By similarity). Interacts with BCL2L11 (By similarity). Homodimer. Heterodimerizes with YWHAE. Homo- and heterodimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with BRAF. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. Interacts with GAB2 and TLK2. Interacts with the 'Thr-369' phosphorylated form of DAPK2. Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with ZFP36L1 (via phosphorylated form); this interaction occurs in a p38 MAPK- and AKT-signaling pathways (By similarity). Interacts with SLITRK1. Interacts with AK5, LDB1, MADD, MARK3, PDE1A and SMARCB1.


Belongs to the 14-3-3 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.


1). KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ. Cancer cell international, 2023 (PubMed: 37848941) [IF=5.8]

Application: WB    Species: Human    Sample: Huh7 cells

Fig. 7 KHDRBS3 binds to YWHAZ and upregulates its expression. (A) Relative mRNA levels of c-Myc, GLUT1, LDHA, YWHAZ, HOXB9, CRABP1 and DLL4 in stable KHDRBS3 knockdown or overexpressing Huh7 cells. (B) Protein levels of c-Myc, GLUT1, LDHA and 14-3-3ζ in infected Huh7 cells were evaluated by western blotting. (C) RIP assay was performed to detect the enrichment of c-Myc, GLUT1, LDHA and YWHAZ in the anti-KHDRBS3 IP group. (D) RNA pull-down assay determined the binding between KHDRBS3 and YWHAZ. Error bars represent standard deviation. **P 

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