EQTN Antibody - #DF15102

Product: EQTN Antibody
Catalog: DF15102
Description: Rabbit polyclonal antibody to EQTN
Application: ELISA(peptide)
Reactivity: Human, Mouse
Mol.Wt.: 33kD(Calculated).
Uniprot: Q9NQ60

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
EQTN Antibody detects endogenous levels of EQTN.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acrosome formation-associated factor; Afaf; AFAF_HUMAN; C9orf11; chromosome 9 open reading frame 11; equatorin;

Immunogens

Immunogen:

A synthesized peptide derived from human EQTN.

Uniprot:

Q9NQ60(EQTN_HUMAN) >>Visit HPA database.

Gene(ID):
EQTN(54586)
Expression:
Q9NQ60 EQTN_HUMAN:

Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.

Sequence:
MNFILFIFIPGVFSLKSSTLKPTIEALPNVLPLNEDVNKQEEKNEDHTPNYAPANEKNGNYYKDIKQYVFTTQNPNGTESEISVRATTDLNFALKNDKTVNATTYEKSTIEEETTTSEPSHKNIQRSTPNVPAFWTMLAKAINGTAVVMDDKDQLFHPIPESDVNATQGENQPDLEDLKIKIMLGISLMTLLLFVVLLAFCSATLYKLRHLSYKSCESQYSVNPELATMSYFHPSEGVSDTSFSKSAESSTFLGTTSSDMRRSGTRTSESKIMTDIISIGSDNEMHENDESVTR

Research Backgrounds

Function:

Acrosomal membrane-anchored protein involved in the process of fertilization and in acrosome biogenesis.

PTMs:

Highly N- and O-glycosylated; contains sialic acid.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle>Acrosome membrane>Single-pass type I membrane protein. Cytoplasmic vesicle>Secretory vesicle>Acrosome inner membrane>Single-pass type I membrane protein. Cytoplasmic vesicle>Secretory vesicle>Acrosome outer membrane>Single-pass type I membrane protein.
Note: In the anterior acrosome region, enriched on the inner acrosomal membrane but minimal on the outer acrosomal membrane; in contrast in the posterior acrosome region enriched on both the inner and outer acrosomal membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.

Subunit Structure:

Interacts with SNAP25.

Restrictive clause

 

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