Product: UBE1L Antibody
Catalog: AF0288
Description: Rabbit polyclonal antibody to UBE1L
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Rabbit
Mol.Wt.: 112kDa; 112kD(Calculated).
Uniprot: P41226
RRID: AB_2833456

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(85%), Rabbit(85%)
Clonality:
Polyclonal
Specificity:
UBE1L Antibody detects endogenous levels of total UBE1L.
RRID:
AB_2833456
Cite Format: Affinity Biosciences Cat# AF0288, RRID:AB_2833456.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D8; MGC12713; UBA1, ubiquitin activating enzyme E1 homolog B; UBA1B; UBA7; UBA7, ubiquitin activating enzyme E1; UBA7_HUMAN; Ube 1L; UBE 2; UBE1L; UBE2; Ubiquitin activating enzyme 2; Ubiquitin activating enzyme E1 homolog; Ubiquitin Activating Enzyme E1 Like; Ubiquitin activating enzyme E1 related protein; Ubiquitin-activating enzyme 7; Ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme-2; ubiquitin-like modifier activating enzyme 7; Ubiquitin-like modifier-activating enzyme 7;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P41226 UBA7_HUMAN:

Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

Description:
UBE1L Activates ubiquitin by first adenylating with ATP its C- terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Belongs to the ubiquitin-activating E1 family.
Sequence:
MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
85
Pig
85
Horse
77
Dog
73
Bovine
62
Chicken
55
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41226 As Substrate

Site PTM Type Enzyme
K8 Ubiquitination
Y15 Phosphorylation
S24 Phosphorylation
K134 Ubiquitination
K257 Ubiquitination
S266 Phosphorylation
K330 Ubiquitination
K429 Ubiquitination
S485 Phosphorylation
K492 Ubiquitination
Y514 Phosphorylation
Y524 Phosphorylation
Y555 Phosphorylation
K696 Ubiquitination
S879 Phosphorylation
S884 Phosphorylation
Y892 Phosphorylation
Y896 Phosphorylation

Research Backgrounds

Function:

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.

PTMs:

ISGylated.

Tissue Specificity:

Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

Subunit Structure:

Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.

Family&Domains:

Belongs to the ubiquitin-activating E1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

References

1). Heme oxygenase-1 prevents heart against myocardial infarction by attenuating ischemic injury-induced cardiomyocytes senescence. EBioMedicine (PubMed: 30527623) [IF=11.1]

2). HSP90AA1 interacts with CSFV NS5A protein and regulates CSFV replication via the JAK/STAT and NF-κB signaling pathway. Frontiers in Immunology (PubMed: 36405689) [IF=7.3]

Application: WB    Species: pig    Sample: PK-15 cell

Figure 8 CSFV infection antagonizes the activation of HSP90AA1 on JAK/STAT pathway. (A, B) Western blot for JAK1, STAT1, p-JAK1, p-STAT1, OAS2, ISG-15 and HSP90AA1 expression in HSP90AA1-overexpression or knock-downed PK-15 and 3D4/2 cells. Cells were infected with CSFV (MOI=1) after transfection p3×Flag-HSP90AA1 or siHSP90AA1. The cells were not infected with CSFV as a control. Cells were harvested at 24hpi and 48hpi served to Western blot. The relative levels of proteins were estimated by histograms representing density reading of the gel bands with Image J, and the ratios were calculated relative to tubulin control. (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 calculated using two-way ANOVA, ns, not significant).

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