Product: SELENOK Antibody
Catalog: DF15288
Description: Rabbit polyclonal antibody to SELENOK
Application: IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 11kD(Calculated).
Uniprot: Q9Y6D0

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
SELENOK Antibody detects endogenous levels of SELENOK.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HSPC030; HSPC297; Selenoprotein K; SelK; SELK_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human SELENOK.

Uniprot:
Gene(ID):
Expression:
Q9Y6D0 SELK_HUMAN:

Highly expressed in heart.

Sequence:
MVYISNGQVLDSRSQSPWRLSLITDFFWGIAEFVVLFFKTLLQQDVKKRRSYGNSSDSRYDDGRGPPGNPPRRMGRINHLRGPSPPPMAGGUGR

Research Backgrounds

Function:

Required for Ca(2+) flux in immune cells and plays a role in T-cell proliferation and in T-cell and neutrophil migration (By similarity). Involved in endoplasmic reticulum-associated degradation (ERAD) of soluble glycosylated proteins. Required for palmitoylation and cell surface expression of CD36 and involved in macrophage uptake of low-density lipoprotein and in foam cell formation (By similarity). Together with ZDHHC6, required for palmitoylation of ITPR1 in immune cells, leading to regulate ITPR1 stability and function. Plays a role in protection of cells from ER stress-induced apoptosis. Protects cells from oxidative stress when overexpressed in cardiomyocytes.

PTMs:

Cleaved by CAPN2/m-calpain in resting macrophages but not in activated macrophages. Macrophage activation up-regulates expression of the calpain inhibitor CAST/calpastatin, resulting in inhibition of CAPN2 activity (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Cell membrane>Single-pass membrane protein.
Note: Probably mainly localized in the ER.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart.

Subunit Structure:

Interacts with DERL1, DERL2, DERL3 and SELENOS. The SELENOK-SELENOS complex interacts with VCP. Interacts with ZDHHC6.

Family&Domains:

Belongs to the selenoprotein K family.

Restrictive clause

 

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