ZP3 Antibody - #DF15626

(3)  
Product: ZP3 Antibody
Catalog: DF15626
Description: Rabbit polyclonal antibody to ZP3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 47kD(Calculated).
Uniprot: P21754

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
ZP3 Antibody detects endogenous levels of ZP3.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HUMZP3; Processed zona pellucida sperm-binding protein 3; Sperm receptor; zona pellucida glycoprotein 3 (sperm receptor); Zona pellucida glycoprotein 3; zona pellucida glycoprotein 3A (sperm receptor); Zona pellucida glycoprotein ZP3; Zona pellucida protein C; Zona pellucida sperm binding protein 3 precursor; Zp-3; ZP3 372; ZP3 424; ZP3 474; ZP3; ZP3_HUMAN; ZP3A; ZP3A/ZP3B; ZP3B; ZPC;

Immunogens

Immunogen:

A synthesized peptide derived from human ZP3.

Uniprot:

P21754(ZP3_HUMAN) >>Visit HPA database.

Gene(ID):
ZP3(7784)
Expression:
P21754 ZP3_HUMAN:

Expressed in oocytes (at protein level).

Sequence:
MELSYRLFICLLLWGSTELCYPQPLWLLQGGASHPETSVQPVLVECQEATLMVMVSKDLFGTGKLIRAADLTLGPEACEPLVSMDTEDVVRFEVGLHECGNSMQVTDDALVYSTFLLHDPRPVGNLSIVRTNRAEIPIECRYPRQGNVSSQAILPTWLPFRTTVFSEEKLTFSLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPTPDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFANDSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCCNKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGPLIFLDRRGDHEVEQWALPSDTSVVLLGVGLAVVVSLTLTAVILVLTRRCRTASHPVSASE

Research Backgrounds

Function:

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

PTMs:

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.

N-glycosylated.

O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.
Note: Located in oocyte zona pellucida.

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in oocytes (at protein level).

Subunit Structure:

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers (By similarity). Interacts with ZP1 and ZP2.

Family&Domains:

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Belongs to the ZP domain family. ZPC subfamily.

Restrictive clause

 

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