Product: SMC1 Antibody
Catalog: AF6439
Description: Rabbit polyclonal antibody to SMC1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Dog, Xenopus
Mol.Wt.: 145kDa; 143kD(Calculated).
Uniprot: Q14683
RRID: AB_2835264

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Xenopus(91%)
SMC1 Antibody detects endogenous levels of total SMC1.
Cite Format: Affinity Biosciences Cat# AF6439, RRID:AB_2835264.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Chromosome segregation protein SmcB; DXS423E; KIAA0178; MGC138332; Sb1.8; Segregation of mitotic chromosomes 1; SMC protein 1A; SMC-1-alpha; SMC-1A; SMC1 (structural maintenance of chromosomes 1 yeast) like 1; SMC1; SMC1 structural maintenance of chromosomes 1 like 1; SMC1A; SMC1A_HUMAN; SMC1alpha; SMC1L1; SMCB; Structural maintenance of chromosomes 1A; Structural maintenance of chromosomes protein 1A;


Smc1 ia a component of the cohesin multiprotein complex that is required for sister chromatid cohesion. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Thought to be an important part of functional kinetochores.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14683 As Substrate

Site PTM Type Enzyme
K5 Ubiquitination
K13 Ubiquitination
K16 Ubiquitination
T28 Phosphorylation
K52 Ubiquitination
K72 Ubiquitination
S85 Phosphorylation
R92 Methylation
Y105 Phosphorylation
K106 Acetylation
K106 Ubiquitination
K110 Ubiquitination
Y117 Phosphorylation
K123 Ubiquitination
K149 Methylation
T152 Phosphorylation
R160 Methylation
S161 Phosphorylation
Y168 Phosphorylation
K170 Ubiquitination
K177 Ubiquitination
T182 Phosphorylation
Y186 Phosphorylation
K189 Ubiquitination
K190 Ubiquitination
K213 Ubiquitination
K237 Ubiquitination
K240 Ubiquitination
K282 Acetylation
S289 Phosphorylation
K294 Ubiquitination
K300 Ubiquitination
S318 Phosphorylation
Y326 Phosphorylation
S358 Phosphorylation
S360 Phosphorylation
T366 Phosphorylation
K373 Ubiquitination
K395 Ubiquitination
K401 Ubiquitination
R406 Methylation
K433 Ubiquitination
K437 Acetylation
K437 Ubiquitination
Y441 Phosphorylation
K446 Ubiquitination
K454 Methylation
K467 Ubiquitination
K475 Ubiquitination
K500 Ubiquitination
K508 Ubiquitination
Y511 Phosphorylation
K528 Ubiquitination
Y530 Phosphorylation
K536 Acetylation
K536 Ubiquitination
K540 Acetylation
K540 Ubiquitination
K561 Ubiquitination
Y575 Phosphorylation
K579 Acetylation
K579 Ubiquitination
K584 Ubiquitination
Y600 Phosphorylation
K607 Ubiquitination
Y611 Phosphorylation
K637 Ubiquitination
T644 Phosphorylation
K648 Acetylation
K648 Ubiquitination
S653 Phosphorylation
S657 Phosphorylation
K660 Ubiquitination
K669 Ubiquitination
K686 Ubiquitination
R699 Methylation
S703 Phosphorylation
K713 Acetylation
K713 Methylation
K713 Ubiquitination
Y714 Phosphorylation
S715 Phosphorylation
S717 Phosphorylation
T722 Phosphorylation
K723 Ubiquitination
K734 Ubiquitination
S735 Phosphorylation
K736 Ubiquitination
K805 Ubiquitination
K806 Ubiquitination
K814 Ubiquitination
T841 Phosphorylation
K874 Ubiquitination
K881 Ubiquitination
K903 Acetylation
K903 Ubiquitination
K910 Acetylation
K918 Ubiquitination
K934 Ubiquitination
K939 Ubiquitination
K944 Ubiquitination
T946 Phosphorylation
S951 Phosphorylation
S956 Phosphorylation
S957 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
S962 Phosphorylation
S964 Phosphorylation
S966 Phosphorylation Q13315 (ATM)
S970 Phosphorylation
S971 Phosphorylation
Y973 Phosphorylation
K1000 Ubiquitination
K1009 Ubiquitination
K1026 Ubiquitination
K1030 Ubiquitination
S1033 Phosphorylation
K1037 Ubiquitination
T1041 Phosphorylation
K1050 Ubiquitination
K1056 Ubiquitination
K1063 Ubiquitination
K1120 Ubiquitination
S1129 Phosphorylation
K1133 Methylation
K1196 Ubiquitination
K1214 Ubiquitination
T1217 Phosphorylation
T1221 Phosphorylation
K1222 Ubiquitination

Research Backgrounds


Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.


Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.

Subcellular Location:

Nucleus. Chromosome. Chromosome>Centromere>Kinetochore.
Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a heterodimer with SMC3 in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their SMC hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. In germ cell cohesin complexes, SMC1A is mutually exclusive with SMC1B (By similarity). Interacts with BRCA1. Found in a complex with CDCA5, SMC3 and RAD21, PDS5A/SCC-112 and PDS5B/APRIN. Interacts with NDC80). Interacts with BRAT1. Found in a complex containing POLE and SMC3. Interacts with RPGR, STAG3 and SYCP2 (By similarity). Found in a cohesin complex with SMC3, STAG1 and RAD21. The SMC1A-SMC3 heterodimer interacts with the NIPBL-MAU2 heterodimer.


The flexible SMC hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure (By similarity).

Belongs to the SMC family. SMC1 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

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