STAT4 Antibody - #AF6441

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Product: STAT4 Antibody
Catalog: AF6441
Description: Rabbit polyclonal antibody to STAT4
Application: WB IHC IF/ICC IP
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Chicken
Mol.Wt.: 86kDa; 86kD(Calculated).
Uniprot: Q14765
RRID: AB_2835265

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Rabbit(100%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
STAT4 Antibody detects endogenous levels of total STAT4.
RRID:
AB_2835265
Cite Format: Affinity Biosciences Cat# AF6441, RRID:AB_2835265.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Signal transducer and activator of transcription 4; SLEB11; STAT4; STAT4_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human STAT4, corresponding to a region within C-terminal amino acids.

Uniprot:

Q14765(STAT4_HUMAN) >>Visit HPA database.

Gene(ID):
STAT4(6775)
Description:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
Sequence:
MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGRVSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLEEQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKLPELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Rabbit
100
Chicken
89
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.

PTMs:

Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the transcription factor STAT family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

References

1). Oncostatin M Induces IFITM1 Expression to Inhibit Hepatitis B Virus Replication Via JAK-STAT Signaling. Cellular and molecular gastroenterology and hepatology, 2024 (PubMed: 37879404) [IF=7.1]

Application: WB    Species: human    Sample: HepG2.2.15 cells

Figure 5. OSM suppresses HBV replication through the JAK-STAT signaling pathway. (A) Extracts from HepG2.2.15 cells treated with OSM (20 ng/mL) for 0∼60 minutes were analyzed for STAT activation. (B) HepG2.2.15 cells were pretreated with ruxolitinib (1 μM) and the AG490 (10 μM) for 1 hour and then were treated with OSM (20 ng/mL) for 10 minutes. The phosphorylation levels of STAT1, STAT3, and STAT5 were examined by immunoblotting. (C, E) The supernatant HBsAg and HBV DNA levels were examined in the indicated groups. One-way analysis of variance. (D) The inhibition effects of OSM on intracellular HBV RNAs and HBV antigens were analyzed in HepG2.2.15 cells pretreated with or without ruxolitinib. Inhibition ratios of OSM for intracellular pgRNA and total RNA for the indicated cell lines were calculated as (1 - OSM-treatment/sham-treatment) ×100. Relative gray values of HBsAg and HBcAg were analyzed by Image J and normalized to β-actin. Unpaired t test. (F) The expression levels of IRFs in HepG2.2.15 cells treated with OSM were examined by quantitative polymerase chain reaction and immunoblotting. Unpaired t test. (G) STAT1-IRF9 heterodimer formation was examined by coimmunoprecipitation in Huh7-1.3 cells treated with OSM. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.
2). Hyocholic Acid Species as the Key Modulator for Cecal Epithelial Homeostasis in Low-Birth-Weight Piglets. Nutrients, 2025 (PubMed: 41228488) [IF=5.9]

Application: WB    Species: piglets    Sample:

Figure 7. Hyocholic acid species (HCAs) improve cecal mucosa barrier function in low-birth-weight piglets. Proteins expression of (a) Wnt family member 8B (WNT8B); (b) interleukin-1β (IL-1β) and signal transduction and activator of transcription-4 (STAT-4); (c) bile acid main receptors G protein-coupled bile acid receptor (GPBAR1) and farnesoid x receptor (FXR); (d) tight junction proteins including Occludin, Claudin-1, Claudin-4, and zonula occludens-1 (ZO-1). (e) Representative immunofluorescence images of mucin 2 (MUC-2, red) and the nuclei stained with DAPI (blue). Full-length blots are presented in the Supporting Information, n = 6 per group.

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