Product: PKD1/PKC mu Antibody
Catalog: AF6443
Description: Rabbit polyclonal antibody to PKD1/PKC mu
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 115kDa; 102kD(Calculated).
Uniprot: Q15139
RRID: AB_2835267

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)
PKD1/PKC mu Antibody detects endogenous levels of total PKD1/PKC mu.
Cite Format: Affinity Biosciences Cat# AF6443, RRID:AB_2835267.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


KPCD1_HUMAN; nPKC D1; nPKC mu; nPKC-D1; nPKC-mu; nPKCD1; nPKCmu; PKC; PKC MU; PKCM; PKCmu; PKD 1; PKD; PKD1; PRKCM; PRKD 1; Prkd1; Protein kinase C mu; Protein kinase C mu type; Protein kinase D; Protein kinase D1; Serine/threonine protein kinase D1; Serine/threonine-protein kinase D1;


Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15139 As Substrate

Site PTM Type Enzyme
Y95 Phosphorylation P12931 (SRC)
S205 Phosphorylation Q15139 (PRKD1) , P17612 (PRKACA) , P98161 (PKD1)
S208 Phosphorylation O15264 (MAPK13)
T210 Phosphorylation
T217 Phosphorylation
S219 Phosphorylation
S223 Phosphorylation
T224 Phosphorylation
S225 Phosphorylation
S235 Phosphorylation
S237 Phosphorylation
S239 Phosphorylation
S247 Phosphorylation
S249 Phosphorylation O15264 (MAPK13)
K292 Ubiquitination
S345 Phosphorylation
S379 Phosphorylation
T395 Phosphorylation
S397 Phosphorylation O15264 (MAPK13)
T400 Phosphorylation
S401 Phosphorylation O15264 (MAPK13)
S412 Phosphorylation Q05655 (PRKCD)
S421 Phosphorylation P17612 (PRKACA)
T422 Phosphorylation
Y432 Phosphorylation A0A173G4P4 (Abl fusion) , P12931 (SRC) , P00519 (ABL1)
Y443 Phosphorylation
S448 Phosphorylation
S460 Phosphorylation
Y463 Phosphorylation P12931 (SRC) , A0A173G4P4 (Abl fusion) , P00519 (ABL1)
S473 Phosphorylation
Y502 Phosphorylation P12931 (SRC) , A0A173G4P4 (Abl fusion) , P00519 (ABL1)
S548 Phosphorylation
S549 Phosphorylation
K612 Acetylation
K622 Ubiquitination
K675 Ubiquitination
K708 Ubiquitination
K724 Ubiquitination
K737 Ubiquitination
S738 Phosphorylation O94806 (PRKD3) , P24723 (PRKCH) , Q15139 (PRKD1) , P17252 (PRKCA) , Q05655 (PRKCD) , Q02156 (PRKCE) , Q05513 (PRKCZ)
S742 Phosphorylation O94806 (PRKD3) , Q05513 (PRKCZ) , Q05655 (PRKCD) , P24723 (PRKCH) , Q15139 (PRKD1) , O15264 (MAPK13) , Q02156 (PRKCE) , P17252 (PRKCA)
T746 Phosphorylation
Y749 Phosphorylation
S890 Phosphorylation
S892 Phosphorylation
S910 Phosphorylation Q15139 (PRKD1) , P98161 (PKD1)

PTMs - Q15139 As Enzyme

Substrate Site Source
O00206 (TLR4) S790 Uniprot
O14558 (HSPB6) S16 Uniprot
O15273 (TCAP) S157 Uniprot
O15273 (TCAP) S161 Uniprot
O43865 (AHCYL1) S68 Uniprot
O60602 (TLR5) S805 Uniprot
O75628 (REM1) S18 Uniprot
O95863 (SNAI1) S11 Uniprot
O96013 (PAK4) S99 Uniprot
P00533 (EGFR) T678 Uniprot
P00533 (EGFR) T693 Uniprot
P02686-5 (MBP) S162 Uniprot
P04792 (HSPB1) S82 Uniprot
P05412 (JUN) S63 Uniprot
P05549 (TFAP2A) S258 Uniprot
P05549 (TFAP2A) S326 Uniprot
P16144-2 (ITGB4) T1736 Uniprot
P16220 (CREB1) S98 Uniprot
P16220 (CREB1) S133 Uniprot
P19174-1 (PLCG1) S1248 Uniprot
P19429 (TNNI3) S23 Uniprot
P19429 (TNNI3) S24 Uniprot
P22059 (OSBP) S240 Uniprot
P23528 (CFL1) S3 Uniprot
P27986 (PIK3R1) S652 Uniprot
P31749 (AKT1) T308 Uniprot
P31749 (AKT1) S473 Uniprot
P35222 (CTNNB1) T112 Uniprot
P35222 (CTNNB1) T120 Uniprot
P43405 (SYK) S291 Uniprot
P48426 (PIP4K2A) T376 Uniprot
P50552 (VASP) S157 Uniprot
P50552 (VASP) S322 Uniprot
P53367-2 (ARFIP1) S100 Uniprot
P53367 (ARFIP1) S132 Uniprot
Q13671 (RIN1) S292 Uniprot
Q13671 (RIN1) S351 Uniprot
Q13936 (CACNA1C) T1928 Uniprot
Q13936 (CACNA1C) S1981 Uniprot
Q14247 (CTTN) S298 Uniprot
Q14247 (CTTN) S348 Uniprot
Q14896 (MYBPC3) S304 Uniprot
Q15139 (PRKD1) S205 Uniprot
Q15139 (PRKD1) S738 Uniprot
Q15139 (PRKD1) S742 Uniprot
Q15139 (PRKD1) S910 Uniprot
Q15276 (RABEP1) S407 Uniprot
Q7KZI7 (MARK2) S400 Uniprot
Q8NER1 (TRPV1) S117 Uniprot
Q8WUI4 (HDAC7) S155 Uniprot
Q8WUI4-5 (HDAC7) S194 Uniprot
Q8WUI4-5 (HDAC7) S220 Uniprot
Q8WUI4 (HDAC7) S358 Uniprot
Q8WUI4-5 (HDAC7) S397 Uniprot
Q8WUI4 (HDAC7) S486 Uniprot
Q8WYL5 (SSH1) S402 Uniprot
Q8WYL5 (SSH1) S937 Uniprot
Q8WYL5 (SSH1) S978 Uniprot
Q92918 (MAP4K1) S171 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S99 Uniprot
Q92934 (BAD) S118 Uniprot
Q96A00 (PPP1R14A) T38 Uniprot
Q96QB1 (DLC1) S1244 Uniprot
Q99683 (MAP3K5) T838 Uniprot
Q99814 (EPAS1) T324 Uniprot
Q9BST9-2 (RTKN) S435 Uniprot
Q9BST9 (RTKN) S448 Uniprot
Q9NRA0 (SPHK2) S419 Uniprot
Q9NRA0 (SPHK2) S421 Uniprot
Q9UBF8 (PI4KB) S294 Uniprot
Q9ULH0-1 (KIDINS220) S918 Uniprot
Q9UQL6 (HDAC5) S259 Uniprot
Q9UQL6-3 (HDAC5) S260 Uniprot
Q9UQL6 (HDAC5) S498 Uniprot
Q9UQL6-3 (HDAC5) S499 Uniprot
Q9Y3E5 (PTRH2) S5 Uniprot
Q9Y3E5 (PTRH2) S87 Uniprot
Q9Y5P4 (COL4A3BP) S132 Uniprot

Research Backgrounds


Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response. Phosphorylates the epidermal growth factor receptor (EGFR) on dual threonine residues, which leads to the suppression of epidermal growth factor (EGF)-induced MAPK8/JNK1 activation and subsequent JUN phosphorylation. Phosphorylates RIN1, inducing RIN1 binding to 14-3-3 proteins YWHAB, YWHAE and YWHAZ and increased competition with RAF1 for binding to GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). Acts downstream of the heterotrimeric G-protein beta/gamma-subunit complex to maintain the structural integrity of the Golgi membranes, and is required for protein transport along the secretory pathway. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane. May act by activating the lipid kinase phosphatidylinositol 4-kinase beta (PI4KB) at the TGN for the local synthesis of phosphorylated inositol lipids, which induces a sequential production of DAG, phosphatidic acid (PA) and lyso-PA (LPA) that are necessary for membrane fission and generation of specific transport carriers to the cell surface. Under oxidative stress, is phosphorylated at Tyr-463 via SRC-ABL1 and contributes to cell survival by activating IKK complex and subsequent nuclear translocation and activation of NFKB1. Involved in cell migration by regulating integrin alpha-5/beta-3 recycling and promoting its recruitment in newly forming focal adhesion. In osteoblast differentiation, mediates the bone morphogenetic protein 2 (BMP2)-induced nuclear export of HDAC7, which results in the inhibition of HDAC7 transcriptional repression of RUNX2. In neurons, plays an important role in neuronal polarity by regulating the biogenesis of TGN-derived dendritic vesicles, and is involved in the maintenance of dendritic arborization and Golgi structure in hippocampal cells. May potentiate mitogenesis induced by the neuropeptide bombesin or vasopressin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. Plays an important role in the proliferative response induced by low calcium in keratinocytes, through sustained activation of MAPK1/3 (ERK1/2) pathway. Downstream of novel PKC signaling, plays a role in cardiac hypertrophy by phosphorylating HDAC5, which in turn triggers XPO1/CRM1-dependent nuclear export of HDAC5, MEF2A transcriptional activation and induction of downstream target genes that promote myocyte hypertrophy and pathological cardiac remodeling. Mediates cardiac troponin I (TNNI3) phosphorylation at the PKA sites, which results in reduced myofilament calcium sensitivity, and accelerated crossbridge cycling kinetics. The PRKD1-HDAC5 pathway is also involved in angiogenesis by mediating VEGFA-induced specific subset of gene expression, cell migration, and tube formation. In response to VEGFA, is necessary and required for HDAC7 phosphorylation which induces HDAC7 nuclear export and endothelial cell proliferation and migration. During apoptosis induced by cytarabine and other genotoxic agents, PRKD1 is cleaved by caspase-3 at Asp-378, resulting in activation of its kinase function and increased sensitivity of cells to the cytotoxic effects of genotoxic agents. In epithelial cells, is required for transducing flagellin-stimulated inflammatory responses by binding and phosphorylating TLR5, which contributes to MAPK14/p38 activation and production of inflammatory cytokines. May play a role in inflammatory response by mediating activation of NF-kappa-B. May be involved in pain transmission by directly modulating TRPV1 receptor. Plays a role in activated KRAS-mediated stabilization of ZNF304 in colorectal cancer (CRC) cells. Regulates nuclear translocation of transcription factor TFEB in macrophages upon live S.enterica infection (By similarity).


Phosphorylated at Ser-397 and Ser-401 by MAPK13 during regulation of insulin secretion in pancreatic beta cells. Phosphorylated by DAPK1. Phosphorylated at Tyr-95 and by ABL at Tyr-463, which primes the kinase in response to oxidative stress, and promotes a second step activating phosphorylation at Ser-738/Ser-742 by PKRD. Phosphorylated on Ser-910 upon S.enterica infection in macrophages (By similarity).

Subcellular Location:

Cytoplasm. Cell membrane. Golgi apparatus>trans-Golgi network.
Note: Translocation to the cell membrane is required for kinase activation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via N-terminus) with ADAP1/CENTA1. Interacts with MAPK13. Interacts with DAPK1 in an oxidative stress-regulated manner. Interacts with USP28; the interaction induces phosphorylation of USP28 and activated KRAS-mediated stabilization of ZNF304. Interacts with AKAP13 (via C-terminal domain) (By similarity).


Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.


1). Angiogenic function of astragaloside IV in rats with myocardial infarction occurs via the PKD1-HDAC5-VEGF pathway. Experimental and Therapeutic Medicine, 2019 (PubMed: 30906439) [IF=2.7]

Application: IHC    Species: rat    Sample: myocardium

Figure 5.| Effect of AS‑IV on the protein expression of PKD1, HDAC5 and VEGF in rats with myocardial infarction. Immunohistochemistry was performed to detect protein expression. Representative and quantitative immunohistochemistry results are presented in the upper and lower panel. Blue arrows represent positive cells for PKD1 expression, red arrows represent the positive cells for HDAC5 expression and green arrows represent the positive cells for VEGF expression. Data are presented as the mean ± standard deviation (n=6‑8). Magnification, x400; scale bar, 50 µm. **P<0.01 vs. the AS‑IV group; ##P<0.01 vs. the AS‑IV‑CID755673 group; $P<0.05 vs. the Model group; &P<0.05 vs. the CID755673 group. AS‑IV, astragaloside IV; PKD1, protein kinase D1; HDAC5, class IIa histone deacetylase 5; VEGF, vascular endothelial growth factor.

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