MARCH2 Antibody - #DF15760

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Product: MARCH2 Antibody
Catalog: DF15760
Description: Rabbit polyclonal antibody to MARCH2
Application: IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 27kD(Calculated).
Uniprot: Q9P0N8

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
MARCH2 Antibody detects endogenous levels of MARCH2.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ubiquitin protein ligase MARCH2; E3 ubiquitin-protein ligase MARCH2; EC 6.3.2.-; HSPC240; MARCH 2; MARCH II; MARCH-II; march2; MARH2_HUMAN; Membrane associated RING CH protein II; Membrane associated ring finger (C3HC4) 2; Membrane associated RING finger protein 2; Membrane-associated RING finger protein 2; Membrane-associated RING-CH protein II; RING finger protein 172; RNF172;

Immunogens

Immunogen:

A synthesized peptide derived from human MARCH2.

Uniprot:

Q9P0N8(MARH2_HUMAN) >>Visit HPA database.

Gene(ID):
MARCH2(51257)
Expression:
Q9P0N8 MARH2_HUMAN:

Broadly expressed.

Sequence:
MTTGDCCHLPGSLCDCSGSPAFSKVVEATGLGPPQYVAQVTSRDGRLLSTVIRALDTPSDGPFCRICHEGANGECLLSPCGCTGTLGAVHKSCLEKWLSSSNTSYCELCHTEFAVEKRPRPLTEWLKDPGPRTEKRTLCCDMVCFLFITPLAAISGWLCLRGAQDHLRLHSQLEAVGLIALTIALFTIYVLWTLVSFRYHCQLYSEWRKTNQKVRLKIREADSPEGPQHSPLAAGLLKKVAEETPV

Research Backgrounds

Function:

E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Lysosome membrane>Multi-pass membrane protein. Endosome membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Broadly expressed.

Subunit Structure:

Interacts with STX6 (By similarity). Interacts with MARCHF3.

Family&Domains:

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Restrictive clause

 

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For Research Use Only.
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