MARCH5 Antibody - #DF15768

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Product: MARCH5 Antibody
Catalog: DF15768
Description: Rabbit polyclonal antibody to MARCH5
Application: IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 31kD(Calculated).
Uniprot: Q9NX47

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
MARCH5 Antibody detects endogenous levels of MARCH5.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ubiquitin-protein ligase MARCH5; FLJ20445; MARCH-V; MARCH5; MARH5_HUMAN; Membrane-associated ring finger (C3HC4) 5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; Mitochondrial ubiquitin ligase; MITOL; RING finger protein 153; RNF153;

Immunogens

Immunogen:

A synthesized peptide derived from human MARCH5.

Uniprot:

Q9NX47(MARH5_HUMAN) >>Visit HPA database.

Gene(ID):
MARCH5(54708)
Expression:
Q9NX47 MARH5_HUMAN:

Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.

Sequence:
MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRILCGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNYPEQEEA

Research Backgrounds

Function:

Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.

PTMs:

Autoubiquitinated leading to degradation (short half-life).

Subcellular Location:

Mitochondrion outer membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.

Subunit Structure:

Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1.

Family&Domains:

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Restrictive clause

 

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For Research Use Only.
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