KPTN Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

KPTN Antibody - Western blot analysis of extracts from various samples, using KPTN Antibody.

KPTN Antibody - DF15915 at 1/100 staining rat heart tissue by IHC-P.

KPTN Antibody - DF15915 staining Hela cells by IF/ICC.

Product:	KPTN Antibody
Catalog:	DF15915
Description:	Rabbit polyclonal antibody to KPTN
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	48kD(Calculated).
Uniprot:	Q9Y664

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

KPTN Antibody detects endogenous levels of KPTN.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

A synthesized peptide derived from human KPTN.

Uniprot:

Q9Y664(KPTN_HUMAN) >>Visit HPA database.

Gene(ID):

KPTN(11133)

Sequence:

Q9Y664 KPTN_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMGEAAVAAGPCPLREDSFTRFSSQSNVYGLAGGAGGRGELLAATLKGKVLGFRYQDLRQKIRPVAKELQFNYIPVDAEIVSIDTFNKSPPKRGLVVGITFIKDSGDKGSPFLNIYCDYEPGSEYNLDSIAQSCLNLELQFTPFQLCHAEVQVGDQLETVFLLSGNDPAIHLYKENEGLHQFEEQPVENLFPELTNLTSSVLWLDVHNFPGTSRRLSALGCQSGYVRVAHVDQRSREVLQMWSVLQDGPISRVIVFSLSAAKETKDRPLQDEYSVLVASMLEPAVVYRDLLNRGLEDQLLLPGSDQFDSVLCSLVTDVDLDGRPEVLVATYGQELLCYKYRGPESGLPEAQHGFHLLWQRSFSSPLLAMAHVDLTGDGLQELAVVSLKGVHILQHSLIQASELVLTRLRHQVEQRRRRLQGLEDGAGAGPAENAAS

PTMs - Q9Y664 As Substrate

Q9Y664

Site	PTM Type	Source
M1	Acetylation	Uniprot
R38	Methylation	Uniprot
K47	Ubiquitination	Uniprot
K67	Ubiquitination	Uniprot
K88	Ubiquitination	Uniprot
T100	Phosphorylation	Uniprot
S223	Phosphorylation	Uniprot
Y287	Phosphorylation	Uniprot
S361	Phosphorylation	Uniprot
S363	Phosphorylation	Uniprot
S364	Phosphorylation	Uniprot
T375	Phosphorylation	Uniprot

Research Backgrounds

Q9Y664_KPTN_HUMAN

Function:

As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.

Subcellular Location:

Lysosome membrane. Cell projection>Lamellipodium. Cell projection>Stereocilium.
Note: Localization to lysosomes is amino acid-independent (PubMed:28199306). Colocalizes with F-actin (PubMed:24239382).

Subunit Structure:

Part of the KICSTOR complex composed of KPTN, ITFG2, C12orf66 and SZT2. SZT2 probably serves as a link between the other three proteins in the KICSTOR complex and mediates the direct interaction with the GATOR1 complex. May associate with F-actin filaments.

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KPTN Antibody - #DF15915