TMEM74 Antibody - #DF15970
(2)
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
TMEM74 Antibody detects endogenous levels of TMEM74.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Transmembrane protein 74;
Immunogens
Immunogen:
A synthesized peptide derived from human TMEM74.
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q96NL1 TMM74_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELHYLAKKSNQADLCDARDWSSRGLPGDQADTAATRAALCCQKQCASTPRATEMEGSKLSSSPASPSSSLQNSTLQPDAFPPGLLHSGNNQITAERKVCNCCSQELETSFTYVDKNINLEQRNRSSPSAKGHNHPGELGWENPNEWSQEAAISLISEEEDDTSSEATSSGKSIDYGFISAILFLVTGILLVIISYIVPREVTVDPNTVAAREMERLEKESARLGAHLDRCVIAGLCLLTLGGVILSCLLMMSMWKGELYRRNRFASSKESAKLYGSFNFRMKTSTNENTLELSLVEEDALAVQS
Research Backgrounds
Function:
Plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction.
Subcellular Location:
Lysosome membrane>Multi-pass membrane protein. Cytoplasmic vesicle>Autophagosome membrane>Multi-pass membrane protein.
Tissue Specificity:
Expressed in heart, lung, and placenta.
Family&Domains:
Belongs to the TMEM74 family.
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